应用形态学、核型、免疫学和分子生物学方法联合检测急性髓细胞性白血病M_(2b)的研究

The Classification of Morphological Immunological Cytogenetic and Molecular Biology in AML-M2b Patients

对20例急性髓细胞性白血病（AML）M2b亚型进行了形态学、免疫学、细胞遗传学和分子生物学方法（MICM）的联合检测。结果显示，形态学分型确诊率为90％（18／20），免疫学分析示80％（16／20）有髓系抗原表达，具有其典型特征者为40％（8／20）。染色体异常t（8；21）检出率为95％（19／20），采用RTPCR技术检测全部病例均显示AWL1－ETO融合基因阳性，提示在MICM联合诊断中，融合基因检测对AML－M2b的诊断，治疗及微小残留病监测有重要意义。

The at of the mphological , immunological , cytoenetic and molecularbilogy calssifiction in 20 patients with adult acute myeloid leukemia M2b (AML-M2b) were reported . It was found that morphological confirmed diagnosis rate was 90% (18/20). The immunological analysis showed the myeloid antigens expression in 16/20 patients , 40% (8/20) patients showed the characteristic immunophenotype . The 95% (l9/20) chromosome translocation t(8; 21) was detected. TheAML1-ETO fusion gene transcripts of 20 paients with AML-M2b were assayed by using a retrotranscriptase polymerase chain reaction (RTPCR) technique . All cases were PCR positive .The results suggested that detection of AML1-ETO fusion gene was a rapid , accurate , sinsitive indicator in the diagnosis , evaluation of treatment and monitoring of minim residual disease .