大鼠急性鲤醇硫酸酯钠中毒血流动力学研究

Hemodynamic study of acute intoxication by a toxic extract from the grass carp bile

探讨急性鲤醇硫酸酯钠（SCS）中毒早期死亡机制和死因。草鱼胆汁提取物SCS按3.0LD50、4．0LD50和5.0LD50的剂量给Wistar大鼠腹腔注射，观察大鼠血流动力学、心电图及呼吸的影响。结果表明；大鼠腹腔注射SCS后，动脉血压（SAP、DAP和MAP）下降；左心室功能（LVP、LVEDP和±dp／dtmax）降低；心电图示心电不稳，心肌缺血S-T段抬高或T波倒置，心率、呼吸先加快而后逐渐变慢，有明显的剂量-效应关系。表明SCS对心肌有选择毒性作用，其早期死因为心源性休克。实验结果为鱼胆中毒的诊断、防治及其法医学鉴定和鱼胆毒素的利用提供有关资料。

15 wistar rats which were randomly devided into three groups, 3 .0LD50,4. 0LD50 and 5. 0LD50 were given ip. injection of sodium cyprinol sulphate (SCS), a toxic extract from the grass carp bile. The changes of hemodynamics, including arterial blood pressure (SAP、 DAP、 MAP ), left ventricular functions (LVP、 LVEDP、 ±dp/dtmax), EKG and respiratory rate were recorded simultaneously by Polygraph System (MR6000, Nihon Konden) before and 1min、 15min、 30min、 1h、2h、3h、 4h、 5h after injection of SCS. The results showed that the arterial blood pressure (SAP、 DAP、 MAP)was falling with any selected dose; the function of the left ventricle, namely LAP、LAVED、±dp/dt max, also markedly decreased; electrocardial potential was unstable、 ST segment part was high and T-wave was invert in EKG. In the early phase, the heart rate and the respiratory rate increased and in the late phase decreased. There were dose-effcet relationship. Those SCS exerted a select damage effect on the heart and the mechanism of death in the early phase was cardiogenic shock.