同时裂解甲基化气相色谱法快速鉴别人体脂肪及动植物油脂

Rapid identification of fatty acids in human body and animal fats and vegetable oils by simultaneous pyrolysis methylation gas chromatography

采用同时裂解甲基化气相色谱法（SPM－GC），首次分析人体脂肪，并且观察人体脂肪、猪、鸡、牛、羊脂和豆油中7种脂肪酸的组份含量变化。根据7种主要脂肪酸（肉豆蔻酸、棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸和亚麻酸）甲酯的百分含量可有效地进行鉴别。结果表明：通过SPM－GC法分析油脂，可将传统的油脂酯化法时间由2个多小时缩短到1分钟左右。数据C．V．％＜4％，最小检测量为1．Oμg。最佳比例四甲基氢氧化按（TMAH）甲醇液（TMAH：甲醇＝1：10，V／V），可消除油脂中多不饱和脂肪酸的异构化和降解。

This is the first report of identifying human fats, animal fats and vegetable oil by simultaneous pyrolysis methylation gas chromatography (SPM-GC). The results demonstrated that the contents of seven fatty acids in fats of human, pig, chicken, ox, and sheep and soybean oil were quite different. They can be identified by SPM-GC according to the content (% ) of methyl esters in seven fatty acids (myristic acid, hexadecanoic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid and linolenic acid). The results showed that the SPM-GC method can cut two hours of traditional esterifying method down to one minute. The value of C. V. % is less than 4.00 %. The minimal detecting amount is 1 .0μg. It is suggested that the isomerization and the degradation of polyunsaturated fatty acids in oils may be eliminated by choosing the optimal proportions of TMAH to methanol solutions (TMAH: methanol = 1: 10, v/v).