补肾活血汤对制动应激所致卵巢功能减退大鼠的影响

Effect of Bushenhuoxue decoction on ovarian dysfunction induced by repeated immobilization stress in rats

目的：探讨补肾活血汤对制动应激大鼠卵巢功能的保护作用。方法：采用重复制动应激法造成大鼠卵巢功能早期减退模型，造模同时灌胃给予补肾活血汤，以放免法检测大鼠血清中性激素水平，酶免法检测血清中激活素和抑制素水平，观察补肾活血汤对造模大鼠卵巢功能的影响。结果：补肾活血汤对制动应激所致卵巢功能早期减退大鼠有明显的保护作用，其高、中剂量可明显升高模型大鼠血清雌二醇水平（P〈0．05），降低模型大鼠血清中异常升高的激活素水平（P〈0．05），升高抑制素和卵泡抑素水平（P〈0．05～0．01）。结论：补肾活血汤对制动应激所致卵巢功能早期减退大鼠的卵巢功能有明显的保护作用。

Objective ： To study the protective effect of Bushenhuoxue decoction on ovarian dysfunction induced by repeated immobilization stress in female rats. Methods： Repeated immobilization stress was used to induce ovarian dysfunction in female rats. The rats were orally administered with Bushenhuoxue decoction. The ovarian function was evaluated by detecting the levels of gonadal and other hormones. Results： Bushenhuoxue decoction remarkably increased the levels of E2 （ P 〈 0.05 ） , INHB and FS （ P 〈 0.05 - 0.01 ） , and decreased the levels of ACTA in serum （P 〈 0.05） after repeated immobilization stress. Conclusion： Bushenhuoxue decoction exerts a protective effect on ovarian dysfunction induced by repeated immobilization stress in female rats.