中国部分地区铜绿假单胞菌氨基糖苷类修饰酶基因型研究

Genotype Distribution of Aminoglycosides-modifying Enzymes in Clinical Isolates of Pseudomonas aeruginosa in Some Areas of China

目的调查中国部分地区临床分离铜绿假单胞菌氨基糖苷类修饰酶(AMEs)基因型分布。方法收集北京、浙江、江苏及湖北等地区5所医院临床分离的165株铜绿假单胞菌,PCR方法检测5种AMEs基因。结果165株铜绿假单胞菌111株检出AMEs基因,总检出率为67.27%,各种AMEs基因检出率分别为:aac(3)-Ⅱ28.48%、aac(6′)-Ⅰ24.85%、aac(6′)-Ⅱ32.12%、ant(3″)-Ⅰ26.06%和ant(2″)-Ⅰ31.52%,各地区AMEs基因型有较大差异。结论中国部分地区铜绿假单胞菌AMEs基因携带率高;各地区AMEs基因型有较大差异。

OBJECTIVE To investigate the genotype distribution of aminoglycosides-modifying enzymes （AMEs） in clinical isolates of Pseudomonas aeruginosa in some areas of China. METHODS Totally 165 clinical strains of P. aeruginosa were collected from five hospitals in different areas. Five AMEs genes were carried out by PCR. RESULTS Form 165 isolates,111 were positive for AMEs genes（67.27%） and the positive rates of genes of aac（3）-Ⅱ,aac（6＇）-Ⅰ,aac（6＇）-Ⅱ,ant（3″）-Ⅰ and ant（2″）-Ⅰ were 28.48%,24.85%,32.12%,26.06% and 31.52% ,respectively. The genotypes of AMEs were discrepant in the different hospitals. CONCLUSIONS The study showed that the positive rate of AMEs genes is high and discrepant in clinical isolates of P. aeruginosa in the different areas of China.