扩张型心肌病患者外周血单个核细胞白细胞介素-35表达及分泌的初步研究

Expression or secretion of interleukin-35 in the peripheral blood mononuclear cells of dilated cardiomyopathy

目的:观察白细胞介素-35(IL-35)在扩张型心肌病(DCM)患者外周血单个核细胞(PBMCs)中表达和血浆水平变化,探讨其在DCM发病中的作用。方法:选择对象为30例DCM的患者(DCM组),24例心力衰竭的患者(HF组)及30例健康体检者为正常对照组。用逆转录-聚合酶链反应(RT-PCR)测定PBMCS的IL-35特异性亚基EB病毒诱导基因3(EBI3)mRNA的表达。用酶联免疫吸附法(ELISA)检测血浆中IL-35水平。结果:①DCM组PBMCs表达IL-35的EBI3mRNA水平(0.15±0.03)明显降低于HF组(0.34±0.08)及正常对照组(0.33±0.07)(均P<0.01),而HF组及正常对照组间差异无统计学意义(P>0.05);②DCM组(24.08±4.40)血浆IL-35的水平明显低于HF组(53.03±5.04)和正常对照组(43.89±8.01)(均P<0.01),而HF组及正常对照组间差异无统计学意义(P>0.05);③DCM组血浆中IL-35含量与射血分数呈正相关(r=0.449,P<0.05),与心功能NYHA分级呈负相关(r=-0.839,P<0.01)。结论:DCM患者的IL-35表达下调,提示IL-35可能在DCM的免疫发病机制中起了一定作用。

Objective：The aim of this study was to observe the level of interleukin-35（IL-35） in peripheral blood mononuclear cells （PBMCs） with dilated cardiomyopathy （DCM）,and explore the role of IL-35 in human DCM. Method：Thirty cases of patients with DCM（DCM group）,24 cases of heart failure patients（HF group） and 30 cases of normal adults（NC group） were studied. The subunit EBI3 （Epstein-Barr virus -induced gene 3） of IL-35 mRNA expression in PBMCs were detected by Reverse Transcription-Ploymerase Chain Reaction （RT-PCR）. And the level of IL-35 in plasma was measured with Enzyme-linked Immunosorbent Assay （ELISA）. Result：①Compared with NC group （0.33±0.07） and HF group （0.34±0.08）,the expression of EBI3 mRNA was significantly lower in DCM group （0.15±0.03） in PBMCs （P〈0.01）.But there was not different between HF group and NC group （P〉0.05）. ②IL-35 protein level in DCM group （24.08±4.40） was significantly lower compared with NC group （43.89±8.01） and HF group （53.03±5.04）. There was a significant difference （P〈0.01）.But there was not different between HF group and NC group （P〉0.05）. ③The IL-35 protein level were strongly positively correlated with ejection fraction（r=0.449,P0.05） of DCM patients,and were negatively correlated with heart function（r=-0.839,P〈0.01） of DCM patients.Conclusion：The decrease of IL-35 in DCM,indicated that IL-35 may play an important role in the immune pathogenesis in DCM.