小鼠心肌组织3种总RNA提取方法比较

Comparison of three different methods for total RNA isolation from mouse heart tissue

目的:探索一种高效的心肌组织总RNA提取方法,对以后总RNA提取工作进行指导。方法:取小鼠心肌组织,分别采用剪刀剪碎法(剪碎法)、研钵加液氮研磨法(研磨法)、离心柱法(过柱法)对小鼠心肌组织进行总RNA提取,紫外分光光度计定量,聚丙烯酰胺凝胶电泳初步定性。结果与结论:研磨法提取出的总RNA量大,质量可靠,较适合于日常总RNA提取;剪碎法对于一些实验条件较差的地区仍然是一种可取的方法;过柱法对于小量心肌组织的提取较为适合。3种方法均能提取出总RNA。

Objective：To find a efficient method for total RNA isolation from mouse heart tissue. Method：Three methods （ shearing method,grinding method and centrifugal mini column method） were used for isolation of total RNA from mouse heart tissue. The isolated RNA was quantitatively and qualitatively detected by UV spectrophotometer and polyacrylamide gel electrophoretic,respectively. Result and Conclution：Total RNA could be isolated by all of the three methods. Moreover,grinding method was suitable for RNA isolation as a routine method because it could extract large quantities of high-quality RNA,shearing method was simple and could be used in some areas without good laboratories,and centrifugal Mini column method was good to be used for RNA isolation from a small heart tissue.