原子力显微镜对不同方式处理的副流感病毒成像的研究

An Atomic Force Microscopy Study on the Images of Para Influenza Virus under Different Treatment Conditions

用原子力显微镜(atom force microscope,AFM)对不同处理条件下的副流感病毒(para influenza virus,PIV)进行成像研究,观察病毒的不同表面形貌和由表及里的超微结构。透射电镜(transmission electron micro-scope,TEM)提供的二维图像可以观察到病毒有圆形和带状的突起。用AFM对完整的病毒颗粒进行成像,从获得的三维图像中我们观察到呈球形的病毒颗粒表面圆形和带状突起的细部结构,两者相比较,AFM的三维图像能更真实地反映病毒的表面形貌和超微结构。用Tritonx-100处理病毒,可使病毒包膜部分或完全溶解,暴露病毒衣壳,通过图像我们观察到了PIV病毒逐步去除包膜后的不同表面形态结构和超微结构。用SDS处理病毒,能够释放出病毒RNA,用AFM进行成像观察,可见病毒的RNA结构。AFM是能够快速有效地对PIV病毒表面形貌和由表及里的超微结构进行研究的有效工具。

Using atomic force microscope（AFM）,we investigated the images of Para influenza virus（PIV） under different treatment conditions and observed the different appearances of the virus and its ultra-microstructure from the exterior to the interior.From the 2D images under transmission electron microscope（TEM）,we could see that the surfaces of PIV particles exhibited spherical and band-shaped ＇tufts＇;from the 3D images under AFM,we could further observe the whole spherical virus particles and their detailed surfaces,which exhibited round and band-shaped ＇tufts＇.Comparing the images under TEM with those under AFM,we found that the latter could reveal the surface topograph and ultramicrostructure of viruses more truly than did the former.The samples of viruses were treated by Tritonx-100,the lipid envelopes of virions were partly or completely resolved,and then most of their capsids were exposed.We could observe the different appearances of the virions under AFM,the lipid envelopes of which were gradually removed.The samples of viruses were also treated by SDS,and the RNA was released from the virions.From the AFM images,we could see the structure of the RNA.It was thus clear that AFM could be used to investigate the different appearances and ultramicrostructure of viruses rapidly and efficiently.