高强度聚焦超声间断照射对微泡空化效应阻断毫米级兔股动脉血流的增强作用

The Impact of Microbubbles-mediated Intermitten HIFU on Bloodflow in Femoral Artery of Rabbit

探讨不同的高强度聚焦超声(highintensity focused ultrasound,HIFU)照射方式在对微泡空化效应阻断毫米级兔股动脉血流中的效应。将108只兔随机分成单纯HIFU照射组、HIFU联合微泡连续照射组和HIFU联合微泡间断照射组。照射后即日、7 d,用HE染色观察兔股动脉组织学变化,透射电镜观察超微结果变化,数字减影血管造影(digital subtraction angiography,DSA)观察血流变化。实验结果表明:照射后,HIFU联合微泡间断照射组所有兔股动脉内均可见血栓形成,血流中断,动脉内皮细胞局受损,组织内观察到空泡的存在;单纯HIFU照射组、HIFU联合微泡连续照射组所有兔股动脉内无血栓形成,血流连续,超微结构无明显变化。HIFU联合微泡间断照射能使毫米级的兔股动脉在较长的时间内(7 d)血栓形成,血流中断;微泡再灌注增强了空化效应。

This study sought to find out whether the femoral artery of rabbit could be occluded after microbubbles mediated intermitten ultrasound exposure.One hundred and eight rabbits were randomly divided into 3 groups subjected to 3 different factors,namely ultrasound,HIFU combined with Microbubble（the intermitten mode and the continuous mode）.After exposure,HE staining was used for visual inspection of the histopathologic change of femoral artery.Electron microscope was used for observation of the ultramicrostructural change;digital subtraction radiography（DSA） was used for inspections of the change in blood flow.There were thrombi formed in the femoral artery and the blood flow was occluded;the endarterium of the femoral artery of rabbits was damaged,and there was cavitation observed in the intermitten exposure group.In the other two groups,there was no change in the the femoral artery of rabbits.Thrombus could be formed in the femoral artery,and blood flow was occluded after exposure to microbubbles-mediated intermitten HIFU.