阿霉素磁性壳聚糖微球的制备及释放实验

Preparation and Release Exam of Magnetic Chitosan Nano-spheres of Doxorubicin

应用改进的悬浮交联技术制备磁性壳聚糖（chitosan,CS）纳米微球。结果显示：该药物微球基本呈球形,其粒径在200-800 nm之间,并且显示了好的磁响应性。阿霉素（doxorubicin,DOX）为试验药物,DOX和CS以化学键（-N=C-）结合在一起,药物含量在1%-15%（w/w）。pH值的大小对其药物体外释放试验的影响很大,当pH为1、2和4时,7 d内药物的释放从22.0%、13.4%降至4.1%。该微球为pH敏感的磁靶向药物微球。

Magnetic chitosan（CS） nano-spheres were prepared by the modified suspension cross-linking technique.The results demonstrated that the magnetic drug nano-spheres are mainly spherical in form with a size of 200 to 800 nm,and show good magnetic responsivity.Here,Doxorubicin was used as exam drug.Glutaraldehyde connects Doxorubicin to CS by the chemical bond（-N=C-）, and the drug content is in range of 1% to 15%（w/w）.The chemical bond is broken depending on pH,so pH is the important factor for the release of doxorubicin.The doxorubicin release was 22.0%,13.4%,and 4.1% in the space of 7d,when pH was 1,2,4.So the nano-spheres are pH-sensitive magnetic targeting drug micro-spheres.