应用小瓶离心培养法分离检测2009年1—6月广州市成人流感样病例相关病毒

Detection of respiratory viruses from patients with influenza like illness in Guangzhou using centrifugation-enhanced shell vials method between January and June, 2009

目的应用高通量的小瓶离心法快速分离检测广州市成人流感样病例相关病毒，分析广州地区患有ILI的成人呼吸道病毒感染情况，评价其在临床实验室开展快速病毒培养的意义。方法采集2009年1—6月广州市成人流感样病例患者标本652份，应用小瓶离心法（包括MDCK、HEp-2、LLC—MK2和MRC-54种细胞），离心接种后，通过观察CPE、血凝（HA）、血吸附（HAD）及直接免疫荧光法（IF）等方法进行病毒分离鉴定。结果652份成人流感样病例标本中共检出阳性161份，5种病毒病原体，病毒培养阳性率为24．69％，其中甲型流感病毒62株（62／161，38．51％），乙型流感病毒88株（88／161，54．65％），副流感病毒8株（8／161，4．96％），腺病毒2株（2／161，1．24％），呼吸道合胞病毒1株（1／161，0．62％k用小瓶离心培养法发出各种病毒培养报告的平均时间分别为：流感病毒2d、腺病毒3d、副流感病毒3d。结论（1）离心培养法具有快速、灵敏、高通量的特点，对临床实验室诊断病毒具有应用推广价值。（2）在分离的ILI病原体中，流感病毒占主导，乙型流感病毒为优势毒株，白2009年1月延续至6月，并呈逐步增多的态势，出现夏季高峰。

Objective To evaluate the application of high-throughput shell vial assay in a clinical laboratory for detection of respiratory viruses from patients with ILI in Guangzhou between January and June, 2009. Methods Six hundred and fifty-two pharyngeal swab specimens were taken from ILI patents. Centrifugation-enhanced shell vials including 4 cell lines （ MDCK, HEp-2, LLC-MK2 and MRC-5 ） were used for culture of respiratory viruses for 2-3 days. The cultures were identified by observation of cytopathic effect （ CPE）, hemmaglution or hemmadsorption test as well as immunofluorescence staining. Results A total of 161 swab samples （24. 69%, 161/652） were shown to have any one of the 5 common respiratory viruses including influenza A viruses （ 38. 51%, 62/161 ）, influenza B virus （ 54. 65% , 88/161 ）, parainfluenza viruses （4. 96% , 8/161 ） , adenovirus （ 1.24% , 2/161 ） , and respiratory syneytial virus （0. 62% ,1/161 ）. The turnaround time was 2d for influenza viruses, 3d for adenovirus and parainfluenza viruses respectively. Conclusions （1）The shell vial method was effective, rapid and high throughout for the detection of respiratory viruses in clinical laboratories. （2） Influenza viruses were dominant in the swab samples from patients with ILI in Guangzhou between January and June with the highest appearance in the summer influenza B virus was the most common pathogen in patients with ILI in this study.