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冠心病患者介入治疗前后循环内皮祖细胞数量的变化 被引量：3

Changes of circulating endothelial progenitor ceils in patients with coronary heart diseases before and after percutaneous coronary intervention
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摘要 目的观察并比较正常人与不同类型冠心病患者外周血中内皮祖细胞（EPCs）数量的差异，以及不同类型冠心病患者在经皮冠状动脉介入治疗（PCI）前后外周血中EPCs的数量变化。方法将患者分成4组：稳定性心绞痛组、不稳定性心绞痛组、急性ST段抬高型心肌梗死组及正常对照组。利用流式细胞术双色分析法检测对照组以及3组不同类型冠心病患者在PCI术前、术后即刻、术后24h EPCs占外周血有核细胞的百分比，其中EPCs以CD133^＋／VEGFR-2^＋双标记阳性确定。结果PCI术前，外周血中EPCs数量在稳定性心绞痛组为（0．043±0．043）％、不稳定性心绞痛组为（0．014±0．018）％、急性ST段抬高型心肌梗死组为（0．040±0．036）％，均明显低于正常对照组[（0．111％±0．078）％]（均P〈0．01），不稳定性心绞痛组外周血中EPCs的数量明显低于稳定性心绞痛组（P〈0．05）。不稳定性心绞痛组外周血中EPCs的数量在PCI术后24h较术前明显增加[（0．054±0．045）％比（0．014±0．018）％，P〈0．01]。稳定性心绞痛组及急性ST段抬高型心肌梗死组PCI术前、术后EPCs的数量差异无统计学意义。结论外周血中EPCs数量的变化可能与冠心病的病变程度有关。PCI对外周血EPCs数量的影响可能与冠心病类型有关。 Objective To compare circulating endothelial progenitor cells （EPCs） number between normal controls and patients with coronary heart diseases （CHD）, and to explore the influence of percutaneous coronary intervention （PCI） on the number of EPCs in patients with CHD. Methods A total of 48 hospitalized patients with CHD were enrolled and divided into three groups, including stable angina pectoris （SAP） group, unstable angina pectoris （UA） group, acute ST-elevation myocardial infarction （STEMI） group. Patients with normal coronary angiography served as controls. The percentage of EPCs in peripheral blood nucleated cells was measured at admission and immediately after and 24 hours after PCI in CHI） patients by double-color flow cytometry analysis. EPCs were identified with CD133^ ＋/VEGFR-2^ ＋. Results At admission, the percentage of EPCs in peripheral blood nucleated cells was significantly lower in SAP group（0. 043% ±0. 043% ） ,UA group（0. 014% ±0. 018% ）and STEMI group（0. 040% ±0. 036% ） than that in the control group（0. 111% ±0. 078% , all P 〈0.01 ）. The number of EPCs in UA group was significantly lower than that in the SAP group （ P 〈 0. 05 ）. In the UA group, the number of EPCs at 24 hours after PCI （0. 054% ±0. 045% ） was significantly higher than before operation （0. 014% ±0. 018%, P 〈0.01 ） and tended to be higher than the value immediately after PCI （0. 028% ±0. 041%, P 〉0. 05）. The before and after PCI EPCs numbers were similar in SAP and STEMI groups （ all P 〉 0. 05 ）. Conclusion The number of peripheral EPCs in patients with CHD is lower than that in normal subjects and negatively related with severity of coronary heart disease. The number of circulating EPCs increased post PCI in patients with unstable angina pectoris.
作者 罗英饰 钱菊英 张磊 马剑英 黄东 樊冰 葛均波
机构地区 上海市心血管疾病研究所 复旦大学附属中山医院心内科
出处 《中华心血管病杂志》 CAS CSCD 北大核心 2010年第2期126-130,共5页 Chinese Journal of Cardiology
基金 国家自然科学基金资助项目（30671998）
关键词 干细胞 冠状动脉疾病 血管成形术 经腔 经皮冠状动脉 Stem ceils Coronary disease Angioplasty, transluminal, percutaneous coronary
分类号 R541.4 [医药卫生—心血管疾病]
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参考文献24

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共引文献9601

同被引文献57

  • 1崔斌,黄岚,宋耀明,李爱民,晋军,覃军,于学军,耿召华.冠心病患者循环内皮祖细胞与相关危险因素及冠状动脉病变的关系[J].中华心血管病杂志,2005,33(9):785-788. 被引量：31
  • 2孔敬博,王孟赞,孔祥泉,徐清波,肖庆忠,陈双峰.老年稳定型心绞痛患者外周血中内皮祖细胞变化及其影响因素[J].山东医药,2006,46(28):4-6. 被引量：2
  • 3祁学文,刘海峰,薛玉增,孔祥泉,Qingbo Xu,Qingzhong Xiao.血管内皮祖细胞与内皮细胞生长因子在冠状动脉粥样硬化性心脏病状态时的变化[J].中国组织工程研究与临床康复,2007,11(42):8439-8443. 被引量：3
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  • 7肖刚峰,张怀勤.普伐他汀对培养人内皮祖细胞药理作用的影响[J].调查与实验研究,2013,6(51):16-18.
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中华心血管病杂志
2010年 第2期

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