丙烯醛吸入致大鼠肺损伤后的蛋白组表达变化

Changes of Proteomics in the Injured Lung of Adult Rats Subjected to Acrolein Inhalation

目的用蛋白组学方法探讨丙烯醛吸入致大鼠肺损伤后的蛋白组表达变化。方法运用质谱分析技术比较差异蛋白的肽指纹图和氨基酸序列并确定差异蛋白名称,为今后研究与肺损伤有关的功能蛋白提供实验依据。结果在丙烯醛吸入组与生理盐水对照组凝胶图谱中平均检测到545个蛋白点。丙烯醛吸入组与生理盐水对照组比较,检测到7个差异蛋白。其中3个上调蛋白为:酪氨酸3和色氨酸5单加氧酶激活蛋白、磷脂酰肌醇转移蛋白和多聚腺苷结合蛋白。4个明显下调蛋白为:2601和2603波形蛋白、S100钙结合蛋白和细胞内钠通道-3。结论本实验鉴定了丙烯醛吸入所致的大鼠肺损伤后与肺组织变化相关的7个蛋白分子,这些分子可能是与肺损伤有关的重要功能蛋白。

Objective To investigate the proteomics change in injured lung tissues of adult rats subjected to acrolein inhalation for 6 weeks.Methods Two-dimensional electrophoresis was used to define the change of proteomic expresses and mass spectrometry was applied to identify the amino acid sequence in differential expressional proteins in lung tissues between acrolein inhalation group and saline control group.Results A total of 545 protein spots were found in the lung tissues tested.Of the 545 proteins,7 differentially expressed proteins were detected in acrolein inhalation group as compared with saline control group.Among them,tyrosine 3/tryptophan 5-monooxygenase activation protein,phosphatidylinositol transfer protein,poly（A）-binding protein,presented a marked upregulation,while 2601 and 2603 vimentin,phosphatidylinositol transfer protein,and chloride intracellular channel 3 showed a marked downregulation.Conclusion This study provides the crucial evidence that differential expressional proteins may underline the machenism of lung injury after acrolein inhalation in adult rats.