Cu(phen)_2^(2+)和Cu(bpy)_2^(2+)与6-巯基嘌呤、腺嘌呤相互作用的研究

Studies on Interaction of Copper Complexes of Cu(phen) 2+ 2 and Cu(bpy) 2+ 2 with 6 Mercaptopurine and Adenine

在ｐＨ为７．０的磷酸盐缓冲溶液中，用荧光光谱、紫外光谱、电化学及紫外光谱电化学等方法研究了铜配合物与６－巯基嘌呤、腺嘌呤的相互作用．结果表明，Ｃｕ（ｐｈｅｎ）２＋２和Ｃｕ（ｂｐｙ）２＋２与６－巯基嘌呤、腺嘌呤发生了相互作用，但作用程度不同．根据荧光光谱实验数据计算出Ｃｕ（ｐｈｅｎ）２＋２和Ｃｕ（ｂｐｙ）２＋２与６－巯基嘌呤、腺嘌呤的配位比均为１∶１；它们与６－巯基嘌呤作用的配位常数分别为２．２３×１０４Ｌ／ｍｏｌ和６．１１×１０４Ｌ／ｍｏｌ；与腺嘌呤作用的配位常数分别为１．９５×１０４Ｌ／ｍｏｌ和５．１２×１０４Ｌ／ｍｏｌ．电化学实验也获得了相近的结果．

In this paper, the interaction of complexes Cu(phen) 2+ 2 and Cu(bpy) 2+ 2 with 6 mercaptopurine (6 MP) and adenine were studied by fluorescence, UV Vis spectroelectrochemical, and spectroelectrochemical methods. Fluorescence and electrochemical results indicated that the complex ratios of copper complexes with 6 MP and adenine are 1∶1, and the complex constants of Cu(phen) 2+ 2 and Cu(bpy) 2+ 2 with 6 MP are 2.23×10 4 L/mol and 6.11 ×10 4 L/mol, with adenine being 1.91×10 4 L/mol and 5.45×10 4 L/mol respectively. Spectroelectrochemical behavior of the copper complexes changed obviously in the presence of 6 MP.These results indicated that there are strong interactions of copper complexes with 6 MP.