摘要
目的:观察丹蛭降糖胶囊对小剂量链脲佐菌素(STZ)加高热量饮食诱导的实验性糖尿病模型大鼠脂肪组织前脂肪细胞因子-1(preadipocyte factor-1,pref-1)mRNA表达的影响。方法:雄性Wistar大鼠随机分为空白组、模型组、治疗组(马来酸罗格列酮组、丹蛭降糖胶囊组),空白组喂以基础饲料,模型组与治疗组注射小剂量STZ并喂以高热量饲料,提取大网膜脂肪组织总RNA,采用RT-PCR技术扩增pref-1基因片段,检测其表达水平。结果:丹蛭降糖胶囊能降低模型大鼠空腹胰岛素(Fins)含量,降低pref-1 mRNA表达,提高胰岛素敏感指数(ISI)。结论:丹蛭降糖胶囊对实验性糖尿病模型大鼠脂肪分化功能有显著改善作用,提示pref-1可能是其治疗2型糖尿病的一个作用靶点。
Objective：Experimental Study of DanzhiJiangtang Capsule（DJC）＇s Effect on diabetic rats induced by low-dose streptozotocin（STZ） and high-energy diet.Preadipocyte factor-1 mRNA＇s expression was used for observation.Methods：Male Wistar rats were randomly divided into blank group,model group and treatment groups（rosiglitazone maleate group and DJC group）,Normal diet was used to feed the blank group.Model group and treatment groups were injected with STZ and fed high-calorie feed.Total RNA was extracted from omental adipose tissue using RT-PCR amplified pref-1 gene fragment to detect the level of its expression.Results：DJC lowered the FIns content of model rats,reducing the expression of pref-1mRNA to improve the ISI.Conclusion：DJC has the effect of significant improvement for experimental diabetes on rat adipose differentiation.It indicates pref-1 may be a target for the treatment of typeⅡ diabetes.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第3期64-67,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药管理局中医药科学技术研究专项(06-07LP22)
安徽省自然科学基金项目(070413130)
安徽省十一五科技攻关项目(08010302184)
