智脑胶囊对阿尔茨海默病模型大鼠脑组织BDNF及其TrkB影响的实验研究

Experimental Research of Effect on Brain-derived Neurotrophic Factors and Tyrosine Protein Kinase Receptors in the Tissue of Experimental Rat Model of Alzheimer's Disease Treated by Zhinao Capsule

目的:观察智脑胶囊对实验性阿尔茨海默病(AD)模型大鼠脑组织脑源性神经营养因子(BDNF)及其酪氨酸蛋白激酶受体B(TrkB)的影响,探讨智脑胶囊治疗AD的作用机制。方法:雄性Wistar大鼠,随机分为7组,即正常对照组、假手术组、AD模型组、石杉碱甲组、智脑胶囊低、中、高剂量组。参照《大鼠脑立体定位图谱》,选双侧Meynert基底核为注射点,以鹅膏蕈氨酸(IBO)毁损双侧Meynert基底核,制作实验性AD大鼠模型。用ELISA酶联免疫法检测脑组织BDNF及其受体TrkB的含量,并观察其在大鼠脑组织皮层和海马不同部位含量的变化。结果:智脑胶囊能提高模型大鼠脑组织(皮层和海马)BDNF及其受体TrkB的含量,与模型组比较,差异有显著性(P<0.05)。其中智脑胶囊高、中剂量组和石杉碱甲组的提高作用优于智脑胶囊低剂量组。结论:智脑胶囊促进大鼠脑组织皮层和海马神经元合成BDNF及其受体TrkB表达增加是其改善学习记忆的重要作用机制。

Objective：To investigate the effect mechanism of Zhinao Capsule in treating Alzheimer disease and effect on brain-derived neurotrophic factors（BDNF） and tyrosine protein kinase receptors in the tissue of experimental rat model of Alzheimer＇s disease.Methods：Male Wistar rats were divided randomly into 7 groups：（1）normal group;（2）Sodium Chloride group;（3）model group;（4） huperzine A group;（5）low dose group of Zhinao Capsule;（6）middle dose group of Zhinao Capsule;（7） high dose group of Zhinao Capsule.According to ＂Brain stereotaxic atlas of rats ＂,animal models of Alzheimer dementia were established by injecting IBO into Meynert basal nuclear.The content of BDNF and TrkB receptor in brain tissue（cortex and hippocampus） were detected by means of ELISA.Results：Compared with model group,Zhinao Capsule could significantly enhance the content of BDNF as well as its TrkB receptor in the cortex and hippocampus of the rats（P0.05）.The performance of high,middle dose of Zhinao Capsule,and huperzine A treated group in improving the content of BDNF and TrkB in brain tissue was higher than low dose group of Zhinao Capsule（P0.05）.The performance of high,middle dose group of Zhinao Capsule was not higher than that in huperzine A group（P（0.05）.Conclusions： Zhinao Capsule has the function of improving the learing-memory ability in model rats,its function mechanism mainly is that Meynert basal neuclea synthesize acetylcholine,transporting more acetylcholine to cortex and hippocampus.Zhinao Capsule can improve the neurons who synthesize BDNF content and express more TrkB receptors.