活血熄风方对局灶性脑缺血大鼠神经细胞凋亡及基因蛋白蛋白表达的影响

Effects of Huoxue Xifeng Recipe on Neuronal Apoptosis and the Expression of Bcl-2 and Bax Protein after Focal Cerebral Infarction in Rats

目的:观察活血熄风方对局灶性脑缺血模型大鼠神经细胞凋亡及Bcl-2、Bax蛋白表达的影响。方法:Wistar雄性大鼠40只随机分为5组:假手术组、模型组、活血熄风方治疗组(剂量分别为20,10,5 g.kg-1)。用光化学法建立局灶性脑缺血大鼠模型,造模前ig给药7 d,造模后24 h取材,用TUNEL法检测神经细胞凋亡数目,免疫荧光与激光共聚焦技术检测Bcl-2、Bax蛋白表达。结果:与模型组比较,活血熄风方各剂量组TUNEL阳性细胞数明显减少,Bcl-2蛋白表达升高,Bax蛋白表达降低,Bcl-2/Bax比值增高(P<0.01或P<0.05)。结论:活血熄风方具有明显的抗凋亡作用,其作用机制可能与增加Bcl-2蛋白表达,降低Bax蛋白表达,提高Bcl-2/Bax比值有关。

Objective： To observe the effects of Huoxue Xifeng recipe（HXR） on neuronal apoptosis and the expression of Bcl-2 and Bax protein in brain tissue after focal cerebral infarction（FCI） in rats.Methods： 40 Wistar male rats were randomly divided into five groups： sham group,model group,HXR group（20,10,5 g·kg^（-1） as high,moderate and low dose respectively）.The model of FCI was established based on the principle of photo-chemical initiation of thrombosis.Intragastric administration of HXR continued 7 days before modeling and stoped the administration 24 h after the modeling.TUNEL technique was used to examine neuronal apoptosis,immunohistochemical and confocal laser scanning microscope methods to examine the expression level of Bcl-2 and Bax protein in brain tissue.Results： Compared with the model group,the apoptosis of neural cells and the expression of Bax protein significantly decreased in the HXR groups.However,the expression of Bcl-2 protein and Bcl-2/Bax ratio increased significantly（P0.01 or P0.05）.Conclusion： HXR has a action of resisting neuronal apoptosis for FCI in rats,the mechanism of neuroprotection may be related to increase in Bcl-2 expression,decrease in Bax expression and increase in Bcl-2/Bax expression ratio.