GMSE培养基鉴定临床感染好氧性无芽胞革兰阳性杆菌的研究

GMSE Substratum in Identificating G^+ Microbacillary of Oxygenphile in Clinical Infection

目的自行研制一种初步鉴定好氧性无芽胞革兰阳性杆菌的一管多用培养基,借以初筛临床感染中常见的好氧性无芽胞革兰阳性杆菌。方法经接种临床常见130株菌株,35℃24 h培养后在自行研制的同一支培养基中同步观察葡萄糖、甘露醇、水杨素、七叶灵(GMSE)试验结果与单管培养基结果的符合率、鉴定结果符合率及培养基的稳定性。结果该培养基的试验结果与单管培养基结果的符合率100%,鉴定结果符合率100%,培养基在密封性良好的条件下保存6个月后质量稳定,仍可使用。结论该培养基配方组成合理,质量稳定,使用简单,判定容易,具有很好的实用性,可初步鉴定临床感染中常见的库特菌属、李斯特菌属、棒杆菌属、丹毒丝菌、乳杆菌属,有助于提高细菌鉴定工作效率和质量。

OBJECTIVE With a self-trituration,multifunction in monotube substratum to make an assessment of Gram positive microbacillary of oxygenphile initially to preliminarily screen the Gram positive microbacillary of oxygenphile in clinical infection.METHODS 130 clinical strains were incubated after cultivating in 35℃ and 24 hours.The results of the test of glucose,mannitol,salicin,aesculin in the same self-trituration were observed at the sametime.Compared the results of canalis singularis substratum′s coincidence,coincidence and the stability of substratum were assessed.RESULTS The coincidence of results of self-trituration and canalis singularis substratum was about to 100%,The assessment result coincidence was about 100%.The substratum can keep stability for about half a year when be stored in an well-leak tightness condition which can still be used.CONCLUSIONS The compounding is reasonable,the quality is stabilized,Using simply,judging easyly and having commendable practicability which can be used in identificating bacteria in clinical infection.Such as Kurthia,Listeria,Corynebacterium,Erysipelothrix,Bacterium lacticum to increase the efficiency and quality in identification of bacteria.