基于枯草芽孢杆菌微生物传感器的毒性分析

Acute toxicity analysis based on Bacillus subtilis microbial biosensor

采用枯草芽孢杆菌(Bacillus subtilis)为指示生物的微生物传感器毒性分析系统,对重金属(Hg2+、Cu2+、Zn2+、Cr6+、Cd2+、Pb2+和Co2+)、有机污染物[邻氯苯酚(2-CP)、2,4-二氯酚(2,4-DCP)、邻硝基酚(2-NP)、对硝基酚(4-NP)、四环素和十二烷基苯磺酸钠]及石油废水等的生物急性毒性进行分析.结果表明,对数生长后期和稳定期的Bacillussubtilis微生物传感器具有良好的毒性分析性能,Cd2+、Zn2+、Cr6+、Cu2+、Hg2+、Pb2+对Bacillus subtilis的EC50分别为47.3,10.9,14.0,2.6,0.8,100.1mg/L,Co2+的EC30为56.6mg/L,2-CP、2,4-DCP、2-NP、4-NP、四环素和十二烷基苯磺酸钠的EC50分别为559.6,450.8,588.5,487.0,121.3,558.9mg/L,该微生物传感器能真实反映石油废水的毒性情况.

Acute toxicities of heavy metals（Hg^2＋, Cu^2＋, Zn^2＋, Cr^6＋, Cd^2＋, Pb^2＋ and Co^2＋）, organic compounds（2-CP, 2,4-DCP, 2-NP, 4-NP, tetracycline and SDBS）and oil wastewater were determined with Bacillus subtilis microbial biosensor.When Bacillus subtilis was in logarithmic and stationary phase, the microbial biosensor had good performance for acute toxicity analysis, the EC50 of Hg^2＋, Cu^2＋, Zn^2＋, Cr^6＋, Cd^2＋, Pb^2＋, 2-CP, 2,4-DCP, 2-NP, 4-NP, tetracycline and SDBS to Bacillus subtilis were 47.3, 10.9, 14.0, 2.6, 0.8, 100.1, 559.6, 450.8, 588.5, 487.0, 121.3 and 558.9 mg/L, respectively, the EC30 of Co2＋ was 56.6mg/L.The microbial biosensor could reflect the actual toxicity of oil field wastewater.