葛根素对兔急性心肌梗死再灌注后无复流的影响

Effect of Puerarin on No-Reflow After Reperfusion of Acute Myocardial Infarction in Rabbits

目的:评价葛根素防治兔急性心肌梗死(AMI)再灌注后无复流的作用。方法:新西兰大白兔48只随机分为对照组和葛根素小中大剂量组,每组12只。结扎冠状动脉左回旋支90min,松解120min制备急性心肌梗死再灌注模型。再灌注期间,各组分别静滴生理盐水及不同剂量葛根素。再灌注后观察左心室心肌无复流程度、梗死程度及ST段抬高指数。结果:①对照组再灌注后出现了明显的无复流及心肌梗死,无复流程度为(65.6±3.1)%,梗死程度为(92.7±2.2)%。无复流程度在小中大剂量葛根素组分别为(51.1±1.4)%、(41.7±2.7)%、(39.4±2.1)%,梗死程度分别为(81.0±4.3)%、(75.2±4.4)%和(71.9±8.4)%,均小于对照组(P均<0.01)。葛根素中和大剂量组无复流和梗死程度也均小于小剂量组(P均<0.01)。大剂量组无复流程度小于中剂量组(P<0.05)。②再灌注后葛根素小中大剂量组ST段抬高指数分别为0.32±0.04、0.26±0.04和0.24±0.03,均小于对照组的0.59±0.07(P均<0.01)。结论:葛根素可减轻兔AMI再灌注后心肌无复流程度及梗死程度。

Objective ：To evaluate the effect of puerarin in preventing and treating no-reflow after reperfusion of acute myocardial infarction （AMI） in rabbits. Methods ：A total of 48 New Zealand white rabbits were randomly divided into 4 groups, Control group, Low-dose, Middle-dose and High-dose puerarin group, n = 12,in each group. AMI/reperfusion model was created by ligating left circumflex artery for 90 minutes, and followed by intravenous reperfusion of physiological saline, low-dose, middle-dose and high-dose of puerarin for 120 minutes in different group respectively. The degree of no-reflow, myocardial infarction and the ST-segment changes of electrocardiogram （expressed as ∑STI） before and after the treatment were examined. Results ： （ 1 ） After the reperfusion, no-reflow zone was （ 65.6 ± 3.1 ） % and infarction size was （ 92. 7 ± 2. 2 ） % in Control group. In Low-dose, Middle-dose and High-dose pnerarin group, the no-reflow zone was （51.1± 1.4 ） % , （41.7 ± 2. 7 ） % and （ 39.4 ± 2. l ） %, respectively ; and the infarction size was （ 81.0 ± 4. 3 ） %, （ 75.2 ±4.4 ） % and （ 71.9 ± 8.4 ） %, respectively. All those indexes were significantly lower than that in Control group （all P 〈 0. 01 ）. No-reflow Zone and infarction size in Mid- dle-dose and High-dose puerarin group were significantly lower than that in Low-dose puerarin group （P 〈 0. 01 respectively）. No-reflow zone in High-dose pnerarin group was lower than that in Middle-dose puerarin group（ P 〈 0. 05 ）. （ 2 ） As compared with Control group,the ~STI after reperfusion decreased from （0. 59 ±0. 07） to （0. 32 ±0. 04）, （0. 26 ±0. 04） and （0.24±0. 03） in Low-dose, Middle-dose and High-dose pnerarin group, respectively. In three puerarin groups, the ∑ STI was significantly lower than that in Control group （P 〈0.01 ）. Conclusion：Puerarin could prevent and treat no-reflow, and reduce the infarction size after AMI reperfusion in rabbits.