HPCE法测定夏枯草中咖啡酸含量

Determination of Caffeic Acid in Spica Prunellae by HPCE

目的建立高效毛细管电泳法测定夏枯草中咖啡酸的含量。方法采用石英毛细管柱;运行缓冲液：20mmol.L-1的硼砂溶液（pH=9.88）;分离电压：18kV;进样时间：5s;温度：25℃,检测波长：323nm。结果咖啡酸在10.281.6μg.mL-1内线性关系良好;r=0.9992;平均回收率为96.7%,RSD为1.97%。结论该方法简便、快速、准确并且专属性强。

Objective To establish a HPCE method for content determination of caffeic acid in Spica Prunellae.Methods An uncoated fused silica capillary;the running voltage was 18kV;the buffer was 20mmol·L-1 boric acid（pH 9.88）;the wavelength was 323nm.Results The linear range of the calibration curve was 10.281.6μg· mL-1（r=0.9992）;the average recovery was 96.7%,and RSD was 1.97%.Conclusion This method is proved to be simple,rapid,accurate and exclusive.