摘要
采用超声波辅助提取金花茶叶多糖(PCCT),在单因素试验的基础上,采用响应面分析法对PCCT提取工艺进行优化研究,以多糖提取率为考察指标,使用苯酚-硫酸法测定PCCT含量。结果表明,最佳提取工艺条件为水料比46:1(mL/g)、温度87℃、提取时间54min、共提3次,测得PCCT的提取率4.39%,鲜叶PCCT含量66.8mg/g。
Optimal ultrasonic-assisted extraction of polysaccharide from Camellia chrysantha （Hu） Tuyama leaves （PCCT） was investigated by single factor method and central composite design coupled with response surface methodology.In addition,a methodological evaluation was conducted for phenol-sulfuric acid determination of polysaccharide.Results showed that the optimal PCCT extraction conditions were as follows：87 ℃ and water/material ratio 46：1 for a triplicate extraction duration of 54 min each time.Under such conditions,PCCT yield was up to 4.39%.Methodological evaluation demonstrated high precision,good repeatability and less error of developed phenol-sulfuric acid method for polysaccharide determination.Fresh Camellia chrysantha （Hu） Tuyama leaves was determined to contain 66.8 mg/g dry weight of polysaccharide by the method.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第4期53-58,共6页
Food Science
基金
广西科技攻关项目(桂科攻0480005)
广西大学科学技术研究重点基金项目(2003ZD05)
关键词
金花茶叶多糖
提取
响应面分析
含量测定
Camellia chrysantha （Hu） Tuyama leaves
polysaccharide （PCCT）
extraction
response surface analysis （RSA）
content determination
