纤维素酶-微波法提取脐橙皮橙皮苷工艺优化

Cellulase Hydrolysis Followed by Microwave-assisted Extraction for Extracting Hesperidin from Navel Orange Peels

为了优化冰糖脐橙皮中橙皮苷的提取工艺,对纤维素酶-微波法结合提取橙皮苷的提取条件进行探讨。得出的较优工艺条件为:纤维素粗酶用量30mg/g干橙粉,pH5.5,酶解时间90min,酶解温度45℃,微波功率560W,微波时间25s,料液比1:50,乙醇体积分数80%。在此条件下,橙皮苷提取率高达6.130%,相对标准偏差(RSD)为0.28%(n=3),说明该工艺稳定可行。

Cellulase hydrolysis,microwave-assisted extraction and cellulase hydrolysis followed by microwave-assisted extraction were investigated individually for the optimal extraction of hesperidin from ＂Bingtang＂ sweet orange （Citrus sinensis L.Osbeck） peels.Results showed that the optimal hesperidin extraction was based on 90 min cellulase hydrolysis at 45 ℃ and pH 5.5 with an enzyme dose of 3% （calculated on the basis of dry weight of material） followed by extraction with 80% ethanol at a solid/liquid ratio of 1：50 under the assistance of microwave treatment for 25 s at 560 W power.In three replicate extractions using the above process,the average yield of hesperidin was as high as 6.130%,and the relative standard deviation was 0.28%,which demonstrates the applicability of the process for extracting hesperidin from ＂Bingtang＂ sweet orange peels.