树脂法优化大豆皂甙的纯化条件及其结构表征

Macroporous Resin Purification and Structural Characterization of Soybean Saponins

研究大孔吸附树脂对低温脱脂豆粕中大豆皂甙的吸附分离特性。选择4种大孔吸附树脂,通过比较对大豆皂甙的静态与动态的吸附和解吸特征,筛选出最佳吸附剂,并对其吸附动力学特征及解吸条件进行研究。结果表明:AB-8型树脂吸附与洗脱的效果最佳;优化出最佳工艺参数为以2BV去离子水除杂后的体积分数70%乙醇溶液为洗脱溶媒,以0.5BV/h流速进行洗脱,收集洗脱液经减压蒸馏及真空冷冻干燥,得皂甙纯品,测定产品纯度为87.07%,得率1.19%。并对冻干纯品进行傅里叶红外光谱表征,鉴定出产品结构与已知大豆皂甙结构一致且纯度可靠。

To screen an optimal adsorbent for the purification of soybean saponins from low-temperature defatted soybean meal,four types of macroporous resin were compared for the differences in dynamic and static adsorption and desorption performances.AB-8-type macroporous resin was shown to have optimal adsorption and desorption capacities towards soybean saponins.The optimal desorption parameters for the purification of soybean saponins using AB-8-type macroporous resin were investigated,and the structure of purified soybean saponins was characterized by infrared （IR） spectroscopy.Results showed that the optimal elution of soybean saponins from AB-8-type macroporous resin was achieved by washing the resin with 2 BV deionized water followed by eluting with 70% ethanol at a flow rate of 0.5 BV/h.The eluate was concentrated by reduced pressure distillation and vacuum freeze dried and purified soybean saponins with 87.07% purity and 1.19% yield were obtained.In addition,the structure of purified product was confirmed by IR spectroscopy to be consistent with the known structure of soybean saponins.