甘蓝中氟啶脲残留量测定方法的研究

HPLC Determination of Chlorfluazuron Residue in Cabbage (Brassica oleracea L.)

建立氟啶脲在甘蓝中的残留分析方法。样品用乙酸乙酯提取,弗罗里硅土柱净化,高效液相色谱法测定。结果表明:本方法最小检出量为1.2ng,在0.05、0.5、2mg/kg添加水平下,添加回收率在81.95%～100%范围内,变异系数范围为2.37%～6.21%。该方法符合农药残留分析的要求,适于甘蓝中氟啶脲的检测。

A HPLC method was developed for the determination of chlorfluazuron residue in cabbage.Samples were extracted with ethyl acetate and cleaned up on Florisil column prior to HPLC analysis.The limit of detection of the method was 1.2 ng.At three spike levels （0.05,0.5,2 mg/kg）,the recoveries of chlorfluazuron ranged from 81.95% to 100%,with coefficients of variation ranging from 2.37% to 6.21%.The figures of merit of this method can meet the requirements of pesticide residue analysis and therefore benefit its application in the determination of chlorfluazuron residue in cabbage.