基于HPLC指纹图谱和定量分析法评价酸枣仁质量

Quality Assessment of Semen Ziziphi Spinosae by HPLC Fingerprinting and Quantitative Analysis

目的:建立中药酸枣仁高效液相色谱(HPLC)指纹图谱,为科学评价不同产地酸枣仁药材质量提供新方法。方法:采用UltimateTMC18柱(250mm×4.6mm,5μm),乙腈-超纯水梯度洗脱,流速1.0mL/min,检测波长210nm,柱温30℃。通过夹角余弦法和相关系数法计算17批样品的相似度,并对其进行聚类。结果:聚类分析表明,17批不同规格样品被聚为3类,即主产区、一般产区和次品。主产区酸枣仁相似度均高于0.9239,酸枣仁皂苷A含量范围为0.8123～1.1109mg/g。结论:采用HPLC法建立指纹图谱并结合指标成分定量分析的方法可作为酸枣仁质量评价的参考依据。

This study was aimed to establish a HPLC fingerprinting approach for the effective quality assessment of Semen Ziziphi Spinosae.In HPLC analysis,the chromatographic separation was achieved on an UltimateTM C18 column （250 mm×4.6 mm,5 μm） with a mobile phase composed of acetonitrile and ultrapure wate at a flow rate of 1.0 mL/min.In addition,detection wavelength and column temperature were set at 210 nm and 30 ℃,respectively.Vector cosine and correlation coefficient methods were used to calculate similarities of 17 samples of Semen Ziziphi Spinosae and cluster analysis was made for them.Results showed that 10 common fingerprint peaks were identified in the samples by HPLC-ELSD while HPLC-UV detection gave 12 common fingerprint peaks.Cluster analysis revealed that the samples were classified into 3 main categories：geographic origins for superior,medium and inferior quality.Samples of Semen Ziziphi Spinosae with superior quality all exhibited a similarity higher than 0.9239 and a jujuboside A content range of 0.8123 to 1.1109 mg/g.It can be concluded that HPLC fingerprinting combined with quantitative analysis of indicator components can provide experimental data for the quality assessment of Semen Ziziphi Spinosae.