冬瓜中DEHP气相色谱-质谱联用检测方法的建立

GC-MS Determination of Di-2-ethylhexyl Phthalate in Fruits of Benincasa hispida

对冬瓜中邻苯二甲酸二(2-乙基己基)酯(DEHP)的含量测定进行较为系统的研究,建立气相色谱-质谱联用测定冬瓜中DEHP含量的方法。结果表明:方法检出限(RSN=3)为2.65×10-3mg/kg,线性范围为0.0001～1.0mg/mL,加标回收率为87.69%～96.85%,平均相对标准偏差(RSD)为3.16%。该分析方法具有准确度高、选择性好、干扰少等特点,能满足冬瓜中DEHP的分析要求,具有实际的应用检测价值。

Benincasa hispida exhibits strong accumulation capacity towards di-2-ethylhexyl phthalate （DEPH）,which proves to be carcinogenic.In the present study,a gas chromatography coupled with mass spectrometric method （GC-MS） was proposed for the determination of DEPH in fruits of Benincasa hispida,based on optimal DEPH extraction process.The developed standard curve of DEPH exhibited a good linearity over the range of 0.0001 to 1.0 mg/mL.The limit of detection （RS/N=3） was 2.65×10-3 mg/kg.The recoveries for DEPH at 5 spike levels were between 87.69% and 96.85%,with an average RSD of 3.16%.The proposed method,thanks to its high accuracy,good selectivity and less interference,meets the requirements for the analysis of DEHP in fruits of Benincasa hispida so as to have a great application prospect.