毒死蜱残留检测间接竞争ELISA试剂盒的研制

Development of An Indirect ELISA Kit for Detecting Chlorpyrifos Residue in Plant-derived Foods

在多克隆抗体的基础上研制一种适用于检测样品中毒死蜱残留的间接竞争酶联免疫吸附分析(ELISA)试剂盒,并对试剂盒的灵敏度、特异性、精密度、准确度和有效期进行测定。结果表明,研制的试剂盒最低检测限为0.5μg/L,线性检测范围为1.8～1000μg/L,供试样品检测结果的批内、批间变异系数均低于8%,回收率均高于85%。试剂盒在4℃或-20℃条件下至少可保存6个月。

Based on the investigations of artificial coating antigen for chlorpyrifos and polycolonal antibody against chlorpyrifos,an indirect ELISA kit was developed for detecting chlorpyrifos residue in plant-derived foods.In addition,the developed kit was evaluated for sensitivity,specificity,precision,accuracy and expiration date.Results indicated that the limit of detection of the kit was 0.5μg/L and the linear range was between 1.8μg/L and 1000 μg/L.Both intra-assay and inter-assay coefficients of variation were less than 8%.Mean spike recoveries of chlorpyrifos in tap water,lettuce,apple and rice were all more than 85%.The kit stored at 4 ℃ or-20 ℃ exhibited more than 6-month expiration date.