高效液相色谱法测定奶粉中的三聚氰胺及其不确定度分析

Uncertainty Evaluation of HPLC Determination of Melamine in Milk Powderchromatography

建立高效液相色谱法测定奶粉中三聚氰胺结果不确定度评定的数学模型,全面考虑测量过程中的不确定度来源,分别运用最小二乘法对标准曲线拟合的不确定度,以及极差法对测定次数较少时引起的不确定度进行评定。结果表明,取样量2.00mg时,测定奶粉中三聚氰胺含量为(2.00±0.088)mg/kg。本方法适用于高效液相色谱法测定奶粉中三聚氰胺的不确定度评定。

The sources of uncertainty in high performance liquid chromatographic （HPLC） determination of melamine in milk powder were fully analyzed according to the calculation formula of melamine.Each uncertainty component was modeled.Uncertainty evaluation was conducted for fitting of standard curve of melamine and less measurement times by least square method and extreme deviation method,respectively.HPLC determination with uncertainty evaluation by the method gave the （2.00 ± 0.088） mg/kg melamine content in a randomly inspected commercial milk powder （weighed 2.00 mg）.This method proves to be applicable to the uncertainty evaluation of HPLC determination of melamine in milk powder.