HPLC-系统内标法测定桂枝汤中芍药苷、甘草苷、肉桂酸、桂皮醛和甘草酸

Determination of paeoniflorin,liquiritin,cinnamic acid,cinnamic aldehyde,and glycyrrhizic acid in Guizhi Decoction by HPLC mehod with sample's internal standard

目的建立同时测定桂枝汤中多个有效成分的系统内标方法(SIS)。方法以桂枝汤中芍药苷为内标,测定芍药苷与甘草苷、肉桂酸、桂皮醛和甘草酸之间的相对校正因子,利用该相对校正因子计算甘草苷、肉桂酸、桂皮醛和甘草酸,并与外标一点法(ACV)测定结果进行比较,以验证方法的准确性和可行性。结果ACV与SIS测定5批桂枝汤中的5种成分的结果无显著差异(P>0.05),可以准确测定桂枝汤中5种成分。结论SIS方法既能同时测定中药中多个成分的量,又能简化实验操作,有望成为适合中药特点的多指标质量评价新模式。

Objective To establish a new content determination method, sample＇s internal standard, based on simultaneous determination of five components in Guizhi Decoction by HPLC under multiple UV wavelengths. Methods Peoniflorin in Guizhi Decoction was chosen as the internal standard, and the related correction factors of liquiritin, einnamic acid, cinnamic aldehyde, and glycyrrhizic acid compared with peoniflorin were measured. The contents of these four components could be calculated by the related correction factors. This method was named to be ＇sample＇s internal standard＇ （SIS）. The method was evaluated by comparison of the quantitative results between angle cosine value （ACV） and SIS method. Results No significant differences were found in the quantitative results of the five components in five batchs of Guizhi Decoction by ACV and SIS methods （P〉0.05）. Conclusion The SIS method not only can determine multiple index in Chinese materia medica （CMM） simultaneously, but also be simple and accurate. It will be a good quality evaluation pattern for CMM.