稀土元素铈对霍山石斛类原球茎悬浮培养细胞生长和多糖合成的影响

Effect of Rare Earth Element Cerium on Cell Growth and Polysaccharide Production in Protocorm-like Bodies of Dendrobium huoshanense during Suspension Culture

研究外源稀土元素铈对霍山石斛类原球茎悬浮培养细胞生长、多糖合成、培养基中碳氮磷源消耗的动力学特性,以及对胞内糖、胞内氮、胞内磷转化的影响。结果表明:适量的铈能够改善霍山石斛类原球茎对碳氮源的利用,提高胞内糖、胞内氮、胞内磷的含量,从而促进细胞生长和多糖合成。以添加10μmol/L的铈效果最好,细胞的生长速率从0.501g/(L·d)提高到0.649g/(L·d);培养至24d时,每升培养液中类原球茎干质量为25.45g,比对照提高了20.2%,培养至18d多糖总质量浓度达到峰值3.35g/L,是对照的1.53倍。

In this study, the effects of cerium, one kind of rare earth elements, on cell growth, polysaccharide biosynthesis, and utilization of carbon, nitrogen and phosphorus sources in protocorm-like bodies of Dendrobium huoshanense during suspension culture were investigated. Results indicated that cerium could improve the utilization of carbon and nitrogen sources in protocorm-like bodies of Dendrobium huoshanense, significantly increase the contents of intracellular polysaccharides, nitrate and phosphate, and enhance cell growth and polysaccharide biosynthesis. The concentration of cerium was optimized to be 10 μmol/L, which could result in the improvement of cell growth rate from 0.501 to 0.649 g/（L·d）. At this optimal concentration, the maximum dry weight of protocorm-like bodies was up to 25.45 g/L after 24 days of cell culture, which was increased by 20.2% compared with the control. Similarly, the highest production of polysaccharides was reached to 3.35 g/L after 18 days of cell culture, which was 1.53-fold amount of the control.