血水草生物碱致钉螺肝脏蛋白质差异表达分析

Proteome analysis on differential expression of liver proteins from Oncomlania hupensis induced by Eomecon chionanthe alkaloids

目的分析血水草生物碱(ECA)致钉螺肝脏蛋白质表达的变化。方法10mg/LECA浸泡钉螺36h后,解剖活钉螺并收集肝脏,双向电泳分离ECA组和清水对照组钉螺肝脏蛋白质,ImageMaster2D5.0软件分析图谱,筛选差异蛋白质,并用MALDI-TOF-TOF质谱进行鉴定。结果ECA组和清水对照组分别检出(354±110)、(311±12)个蛋白点;质谱鉴定获得假定蛋白、ENSANGP00000022175蛋白、醛脱氢酶、未知蛋白、ENSAN-GP00000027067蛋白、类似锚蛋白3的1亚型4亚亚型蛋白、相似A型肝炎病毒短型细胞受体1的蛋白、线粒体异柠檬酸脱氢酶2样蛋白、类似角蛋白的蛋白、角蛋白10、角蛋白1共11个蛋白质,这些蛋白质均为ECA处理后表达上调的蛋白质。结论ECA可引起钉螺肝脏蛋白质表达发生改变。

Objective To analyze the influence of Eomecon chionanthe alkaloids （ECA） on differential expression of liver proteins from Oncomlania hupensis. Methods O. hupensis was immersed in 10 mg/L ECA or clean water for 36 h and livers were isolated from live snails. Total liver proteins were extracted and separated by two-dimensional gel electrophoresis, differentially expressed proteins between ECA group and clean water group were selected and analyzed by matrix-assisted laser desorption/ionization time-offlight mass spectrometry （MALDI-TOF-MS） and tandem mass spectrometry sequencing of tryptic peptides. Results There were （354±110） and （311±12） spots observed in ECA group and in water group, respectively. Eleven differential expressed proteins were identified and all were up-regulated in ECA group. The eleven identified proteins were hypothetical protein, ENSANGP00000022175, class Ⅰ aldehyde dehydrogenase, unknown protein, ENSANGP00000027067, protein similar to ankyrin 3 isoform 1 isoform 4, protein similar to hepatitis A virus cellular receptor 1 short form, mitochondrial isocitrate dehydrogenase 2-like, protein similar to keratin, keratin-10, and keratin-1, respectively. Conclusion Expression of liver proteins from O. hupensis could be changed when immerse in ECA.