细菌纤维素高产菌株的筛选及鉴定

Screening and Identification of Cellulose-producing Strains

以残次的水果为原料,经静置富集培养、多次筛选纯化等步骤,分离出一株细菌纤维素产量较高且遗传性能稳定的菌株G-29。根据第二版《伯杰系统细菌学手册》和第九版《伯杰氏细菌鉴定手册》,对该菌株进行形态、生理生化特征和16SrDNA分子序列分析。初步鉴定该菌为葡糖醋杆菌属(Gluconacetobactersp.)一个新菌种或者中间葡糖醋杆菌(Gluconacetobacter intermedius)的一个亚种。菌株G-29已保藏于中国典型培养物保藏中心,保藏编号为CCTCCNO:M208234。

Wounded fruits were used as raw materials to isolate and obtain a cellulose-producing strain, G-29, with high productivity and genetic stability through static enriching culture and repeated screening. The morphology, physiological and biochemical characteristics and 16S rDNA sequence of G-29 were analyzed. This strain was identified to be a new subspecies of Gluconacetobacter intermedius in the species of Gluconacetobacter. This strain has been preserved in China Center for Typical Culture Collection （CCTCC） with the collection number of CCTCC NO： M208234.