阿魏萃取物对阿魏菇发酵培养液中3种酶酶活力的影响

Effect of Organic Solvent Extract from Ferula ferulaeoides on Activities of Enzymes in Fermenation Supernatant and Mycelia of Pleurotus ferulae

以阿魏菇NB-1为液体发酵菌种,在发酵培养液中添加阿魏的三氯甲烷萃取物,27.5℃培养7d,测定发酵液中漆酶、多酚氧化酶(PPO)、蛋白酶以及菌丝体中PPO酶活力。结果表明:阿魏三氯甲烷萃取物添加量为10、50、100mg/100mL时,发酵液中漆酶酶活力分别是对照的40、50、50倍;发酵液PPO酶活力分别是对照的4.43、30.14、43.14倍;菌丝体中PPO酶活力分别是对照的1.6、0.93、1.06倍,萃取物对菌丝体PPO酶活力产生了双重作用;发酵液蛋白酶酶活力分别为对照的0.99、0.85、0.67倍,表现出随阿魏三氯甲烷萃取物剂量增加,酶活力降低的趋势。

In this study, Pleurotus ferulae NB-1 was used for liquid fermentation. The chloroform soluble fraction of 95% ethanol extract from Ferula ferulaeoides root was added to fermentation culture medium of the strain. After 7 days of fermentation at 27.5 ℃, the resulting fermentation broth was harvested and separated into supernatant and mycelia by centrifugation, and the activities of laccase, polyphenol oxidase （PPO） and protease in the supernatant and mycelial PPO were determined. Results indicated that the addition of the chloroform soluble fraction of 95% ethanol extract from Ferula ferulaeoides root at the levels of 10, 50 and 100 mg to 100 mL of fermentation culture medium resulted in 40-, 50- and 50-fold increases of laccase activity, 4.43-, 30.14- and 43.14-fold increases of PPO activity and 0.99-, 0.85- and 0.67-fold increase of protease in the supernatant, and 1.6-, 0.93- and 1.06-fold increases of mycelial PPO activity, respectively when compared with no addition. These results demonstrate that the chloroform soluble fraction of 95% ethanol extract from Ferula ferulaeoides root exhibits a promotion effect at low concentration and an inhibition effect on PPO activity at high concentration. Moreover, protease activity exhibits a decreasing trend in a dosedependent manner.