秦艽细胞悬浮培养研究(Ⅰ)

Cell suspension culture of Gentiana macrophylla(Ⅰ)

目的建立秦艽细胞悬浮培养体系。方法通过小细胞团法优选细胞系并考察不同培养基、细胞种龄、初始pH值、培养温度、摇床转速对秦艽悬浮细胞生长及龙胆苦苷积累的影响。结果适于秦艽细胞悬浮培养条件为:MS培养基为基本培养基,用15d种龄的细胞接种,初始pH7、25℃,110r/min,摇瓶培养。结论通过摇瓶培养建立了良好的秦艽细胞悬浮培养体系。不同培养基对细胞生长及龙胆苦苷积累的显著影响提示某些无机离子有重要的调控作用。

Objective To establish cell suspension culture system of Gentiana macrophylla. Methods Good cell line was selected by the methods of small cell aggregate and the effects of different media, inoculation age of cells, initial pH value, culture temperature, and rotation speed on cell growth and gentiopi- croside accumulation were investigated. Results The eligible cell suspension culture conditions were： MS media as the basic medium, cells with inoculation age of 15 d, initial pH 7, and rota＊ion speed of 110 r/min at 25 ℃, culturing in Erlenmeyer flasks. Conclusion Optimal cell suspension culture system of G. macrophylla has been established by shake flasks. The different effects of media on cell growth and gentiopicroside accumulation indicate that some inorganic ions have significant effects on regulation.