冬虫夏草原生质体诱变育种研究

Ultraviolet-induced Protoplast Mutagenesis for Breeding High-performance Strains of Cordyceps sinensis

以冬虫夏草原生质体为材料,经紫外诱变获得了性状优于出发菌株的突变株。实验结果表明:生物量最高的再生菌株为诱变30s的8号菌株,生物量达到1.3445g/100mL,比出发菌株生物量提高55.16%;胞内多糖产量最高的再生菌株为诱变30s的3号菌株,胞内多糖产量达到54.570mg/g,比出发菌株多糖产量提高52.9%;富硒能力较高的突变菌株是诱变30s的8号菌株,富硒量达到125.8μg/g,比出发菌株提高50.11%。由结果可知,原生质体诱变技术可以用于冬虫夏草的菌种选育。

Cordyceps sinensis was used as original strain to conduct protoplast mutagenesis with ultraviolet （UV） illumination for obtaining high-performance mutants. Results showed that 30-second UV illumination gave protoplast-regenerated strains of Cordyceps sinensis No.3 with the highest intercellular polysaccharide production and No.8 with the highest biomass and ability to enrich selenium. The intercellular polysaccharide content （calculated on the basis of dry weight of Cordyceps sinensis mycelia） in strain No. 3 reached up to 54.570 mg/g, which was increased by 52.9 % when compared with that in original strain. Strain No. 8 exhibited 1.3445 g/100mL biomass and 125.8 μg/g selenium content （also calculated on the basis of dry weight of Cordyceps sinensis mycelia）, which were increased by 55.16% and 50.11%, respectively, when compared with original strain.