Beagle犬免疫血清Ⅰ型腺病毒抗体检测

Detection of Serum Antibody in Beagle Dogs Vaccinated with Canine Adenovirus Type 1

目的为Beagle犬犬传染性肝炎(ICH)的预防制定合适的免疫程序提供依据。方法采用间接血凝抑制实验对24头Beagle犬(雌雄各半,20日龄～14周龄)注苗前后120份Beagle犬血清I型犬腺病毒血凝抑制抗体(HI)进行检测。结果注苗前20日龄仔犬血清母源性HI抗体效价为1:8～1∶32(平均1∶20),35日龄仔犬血清母源HI效价下降为1∶2～1∶8(平均1∶5);首免后2w(56日龄)血清HI效价上升为1∶8～1∶32(平均1∶14),二免后3w(77日龄)血清HI抗体效价上升为1∶32～1∶128(平均1∶45),三免后3w(98日龄)血清HI抗体效价继续上升为1∶64～1∶256(平均1∶79)。结论预防Beagle犬犬传染性肝炎(ICH)建议使用FiveStar犬五联弱毒疫苗,首免为35～40日龄,每间隔3w进行二免和三免加强。

Objective To establish a proper procedure for vaccination regimen against infectious canine hepatitis （ICH） in Beagle dogs.Methods Canine hepatitis hemagglutination inhibition （HI） antibody of 120 serum samples collected from 24 Beagle dogs （20-day to 14-week old） was examined by the indirect hemagglutination inhibition test.Results Maternal HI antibody titer was 1∶8-1∶32 （average 1∶20） and 1∶2-1∶8 （average 1∶5） in 20-day old and 35-day old puppy respectively. HI antibody titer was 1∶8-1∶32 （average 1∶14） 3 weeks after the first immunization, and 1∶32-1∶128 （average 1∶45） 3 weeks after the second immunization and 1∶64-1∶256 （average 1∶79） 3 weeks after the third immunization, respectively.Conclusion The Best Combinational Canine Attenuated-live Virus Vaccines are preferred to prevent canine hepatitis, and the first immunization should be performed in 35-40-day old. The second and the third immunization should be performed at an interval of 3 weeks after the first and the second immunization respectively.