VEGF及其受体FLK-1在鼻咽癌中的表达及意义

Expression and clinical significance of VEGF and FLK-1 in Nasopharyngeal Carcinoma

目的研究VEGF及其受体FLK-1在鼻咽癌中的表达,探讨VEGF与鼻咽癌生长的关系,为临床治疗鼻咽癌提供理论依据。方法采用免疫组化SP法检测30例鼻咽黏膜慢性炎和40例鼻咽癌组织中VEGF、FLK-1的表达。结果VEGF和FLK-1在鼻咽黏膜慢性炎中的阳性率分别为20.00%(6/30)和13.33%(4/30);在鼻咽癌中的阳性率分别为82.50%(33/40)和75.00%(30/40),与鼻咽黏膜慢性炎组比较差异均有显著性(P<0.001)。在鼻咽癌中VEGF和FLK-1的表达有显著相关性(r=0.582,P<0.001)。结论在鼻咽癌中,VEGF及其受体FLK-1均呈高表达,VEGF可能通过旁分泌和自分泌方式对鼻咽癌的生长、增殖起着重要的调控作用,抑制VEGF的表达可以是治疗鼻咽癌的一个靶点。

Objective To detect the expression of VEGF and FLK-1 in NPC, and to evaluate the rela- tionships of VEGF with the growth of it,in order to provide the theory for clinical treats NPC. Methods Immu- nohistoehemieal（IHC） methods（ SP）were used to examine the expression of VEGF and FLK-1 in paraffin-em- bedded speeiments of 30 chronic nasopharyngitis and 40 NPC. Results The positive rates of VEGF and FLK-1 protein expression in chronic nasopharyngitis were 20. 00% （6/30）and 13.33 % （4/30）in respective; the posi- tive rates of two protein were 82. 50% （ 33/40 ） and 75.00% （ 30/40 ） in NPC, with significant difference com- pared with chronic nasopharyngitis respectively （P 〈 0. 001 ）. In NPC spearman correlation analysis showed there were significant correlation between the expression on VEGF and FLK-1. Conclusion In NPC, there were obvi- ous positive expression of VEGF and FLK-1, VEGF might have important regulatory effeets on growth and prolif- eration of the nasopharyngeal carcinoma cells by the pathway of autocrine and paraerine, restraining the expres- sion of VEGF eould become a favourable target on the therapy of NPC.