期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

白介素-17对急性肺损伤小鼠中性粒细胞凋亡的影响 被引量：2

Effect of Interleukin-17 on PMN apoptosis of acute lung injury mice
下载PDF
导出
摘要 目的白介素-17(IL-17)作为前炎症因子可以导致趋化性细胞因子的增高,从而促进中性粒细胞和单核细胞的募集。本实验通过建立急性肺损伤(ALI)小鼠模型观察IL-17在体内环境下对中性粒细胞凋亡的影响,探讨体内环境下IL-17的变化与血液中性粒细胞的凋亡的关系。方法雄性C57小鼠30只,体质量22～27g,随机分为正常对照组(对照组),15只;ALI模型组(模型组),15只,脂多糖(LPS)腹腔注射6mg/kg、10ml/kg致急性肺损伤。分别观察小鼠呼吸频率、活动情况及直接光镜观察肺组织血管及肺泡变化情况,中性粒细胞凋亡,淋巴细胞CD3+/4+/8+分类检测,血清IL-17含量的检测。结果急性肺损伤时小鼠的IL-17的含量(0.10±0.002)pg/L,正常对照组(0.08±0.003)pg/L(P<0.05)。CD4+%模型组(21.99±1.83),对照组(14.97±0.99)(P<0.01)。中性粒细胞凋亡百分比(%)模型组(1.15±0.17)%,对照组(0.32±0.05)%(P<0.01)。结论在ALI发病24h时,由于各种炎性细胞因子及其他炎性介质的作用,中性粒细胞表达了较高的凋亡率,而IL-17在其中起到了一定作用,中性粒细胞的凋亡与IL-17有一定的相关性。 Objective As pro-inflammatory cytokine（PIC）,Interleukin（IL-17） can lead the increase of chemoattractant cytokine,which induce the recruitment of PMN and monocytes. Through the setting of ALI mice model in the study,to observe the effect of Interleukin（IL-17） on PMN apoptosis in vivo,and the relation between in vivio change of IL-17 and PMN apoptosis. Methods 30 male C57 mices,with weight of 22 ～ 27 g were divided randomly to normal control group（ control group）,15 mices; ALI model group（ Model group）,10 mices,LPS intraperitoneal injection 6 mg/kg,10 ml/kg was made to induce acute lung injury. Respiratory rate and movement of mice was observered,light microscope observation was made for the change of lung vessels and alveolus,PMN apoptosis testing,Lymphocyte CD3 ＋ /4 ＋ /8 ＋ classified detection and detection of IL-17 concentration in serum was made respectively. Results IL-17concentration in mice with ALI was（0. 10 ± 0. 002） pg/L,normal control group（0. 08 ± 0. 003） pg/L （P〈0. 05）. CD4 ＋ % model group（21. 99 ± 1. 83） control group（14. 97 ± 0. 99）（P〈0. 01）. PMN apoptosis percentage（% ）model group（1. 15 ± 0. 17）% control group （0. 32 ±0. 05）%（P〈0. 01）. Conclusions At 24 hours after ALI occur,percentage of PMN apoptosis is relatively high,due to the effect of various inflammatory cytokines and other inflammatory medium,it is IL-17 that plays a certain role in the process,which indicates that PMN apoptosis is related to IL-17.
作者 李雁 徐红日 聂波 蔡阳平
机构地区 北京中医药大学东直门医院急诊科
出处 《中国实用医药》 2010年第6期9-10,共2页 China Practical Medicine
关键词 急性肺损伤 小鼠 中性粒细胞 凋亡 Acute lung injury Mice Interleukin-17 PMN Apoptosis
分类号 R563.8 [医药卫生—呼吸系统]
  • 相关文献

参考文献7

  • 1Laan M, Cul ZH, Hshino H, et al, Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol, 1999, 162(4) :2347-2352.
  • 2Rahman MS, Yang J, Shan LY, et al. IL-17R activation of human airway smooth muscle cells induces CXCL-8 production via a transcriptional-dependent mechanism. Clin Iminunol, 2005, 115 (3): 268 -276.
  • 3Albanesi C, Cavani A, Girolomonl G, et al. IL-17 is produced by nickelspecific T lymphocytes and regulates ICAM-1 expresion and chemokine production in human keratinovytes:synergistic or antagonist effects with IFN-gamma and TNF-alpha. J Immunol, 1999,162 ( 1 ) :494-502.
  • 4Ye P, Rodriguez FH, Kanaly S, et al. Requirement of interleukin 17 receptor signaling for lung cxc chemokine and granulocyte colonystimulating factor expression, neutrophil recruitment, and host defense. J Exp Med, 2001, 194(4) :519-528.
  • 5Carol E, Jones CE, Chan K, et al. Interleukin-17 stimulates the expression of interleukin-8, growth-related oncogene-α, and granulocye-golony-stimulating factor by human airway epithelial cells. Am J Respir Cell Mol Biol, 2002,26(6) :748-753.
  • 6张志刚,何权瀛,刘新民,汤秀英,陈路增.白细胞介素-17对中性粒细胞凋亡的影响[J].北京大学学报（医学版）,2006,38(3):305-309. 被引量：8
  • 7Hussain IV, Wu F,Zhu L, et al. Neutrophit apoptosis during the development and resolution of oleic acid-induced acute lung injury in the rat. American J of Respir. Cell and Molecular Bio, 1998,19 (6).

二级参考文献11

  • 1Cox G, Austin RC. Dexamethasome-induced suppression of apoptosis in human neutrophils requires continuous stimulation of new protein synthesis[ J]. J Leuk Biol, 1997,61:224-230.
  • 2Lee A, Whyto MKB, Haslett C. Inhibition of apoptosis and prolongation of neutrophil functional longerity by inflammatory mediators[J]. J Leukoc Biol, 1993,54:283 -288.
  • 3Hachiya O, Takeda Y, Miyata H, et al. Inhibition by bacterial lipopolysaccharide of spontaneous and TNF-α-induced human neutrophil apoptosis in vitro[ J ]. Microbiol Immunol, 1995,39:715 -723.
  • 4Takeda Y, Watanabe H, Yonehara S, et al. Rapid acceleration of neutrophil apoptosis by tumor necrosis factor-α [J].Int Immunol,1993,5:691 - 694.
  • 5Jovanovic DV, Di Battista JA, Martel-pelletier J, et al. IL-17 stimulates the production and expression of proinflammatory cytokines, IL-1β and TNF-α, by human macrophages [ J ]. J Immunol, 1998,160:3513-3521.
  • 6Jones CE, Chan K. Interleukin-17 stimulates the expression of interleukin-8, growth-related oncogene-α, and granulocyte colony-stimulating factor by human airway epithelial cells[ J]. Am J Respir Cell Mol Biol, 2002,26:748 -775.
  • 7Laan M, Cui ZH, Hoshino H, et al. Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the alrways[J]. J Immunol, 1999,162:2347 - 2352.
  • 8Ye P, Garvey PB,Zhang P, et al. Interleukin-17 and lung host defense against Klebsiella pneumoniae infection[ J]. Am J Respir Cell Mol Biol, 2001,25:335 -340.
  • 9Bush KA, Walker JS, Lee CS, et al. Cytokine expression and synovial pathology in the initation and spontaneous resolution phases of adjavant arthritis: interleukin-17 expression is upregulated in early disease [ J ]. Clin Exp Immunol, 2001,123:487 - 495.
  • 10Wong CK, Ho CY, Li EK, et al. Elevation of proinflammatory cytokine (IL-18, IL-17, IL-12) and Th2 eytokine (IL-4) concentrations in patients with systemic lupus erythematous [J].Lupus,2000,9:589 - 593.

共引文献7

同被引文献8

引证文献2

二级引证文献3

中国实用医药
2010年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部