螯合剂与牛血清白蛋白竞争镍作用的研究

The Chelating Competition for Bovine Serum Albumin Bound Ni(Ⅱ)

用凝胶色谱法研究了13种螯合剂使牛血清白蛋白镍配合物BSANi(Ⅱ)中的镍活动化的能力,发现DTPA、NTA等氨羧类螯合剂有较好的竞争能力,而巯基类较差。用透析法研究了螯合剂与BSANi(Ⅱ)反应动力学机理,结果表明,在pH7.4和4℃时,L-组氨酸、EDTA为解离机理,而亚氨二乙酸,二乙三氨五乙酸为加合机理。测定了有关的动力学参数,依据竞争能力参数F讨论了可能作为治疗镍中毒的促排螯合剂。

The mobilizing powers of thirteen chelating agents for Bovine Albumin Bound Ni(Ⅱ) (BSANi( Ⅱ)) were studied by gel chromatographic method. The experimental results show that the polyaminopolycarboxylate type chelators, especially DTPA and NTA are stronger mobilizing ligands than the mercapto chelators. The kinetics of these reactions have been studied at pH = 7.4, and the mechanism has been elucidated. The reactions of His and EDTA with BSANi(Ⅱ) were found following the dissociation mechanism, but iminodiace-tic acid (IDA) and DTPA, association mechanism. The kinetic parameters were determined by dialysis method. The potentiality of the chelators as the agents to accelerate the excretion of Ni is discussed on the basis of the F.