鱼腥草减轻糖尿病大鼠肾损害的作用机理研究

Mechanism of Herba Houttuyniae on Relieving Renal Impairment in Streptozotocin-induced Diabetic Rats

目的研究鱼腥草蒸馏液对2型糖尿病大鼠尿蛋白、尿白蛋白(Alb)、肾组织结缔组织生长因子(CTGF)、脂联素、单核细胞趋化蛋白-1(MCP-1)的影响。方法采用高糖高脂饲料喂养,配合小剂量链脲佐菌素(STZ)一次性腹腔注射造成2型糖尿病大鼠模型,经鱼腥草蒸馏液灌胃治疗8周后,观察大鼠24h尿蛋白、Alb、肾组织CTGF、脂联素、MCP-1等指标的变化。结果治疗8周后,鱼腥草蒸馏液能明显改善STZ大鼠多饮、多食和消瘦,降低24h尿蛋白、Alb,减少肾组织CTGF和MCP-1表达。但对脂联素的影响不显著。结论鱼腥草蒸馏液对糖尿病大鼠肾脏病变有一定的改善,其作用机制可能与降低尿蛋白、Alb及肾脏CTGF和MCP-1的表达有关。

Objective To investigate the influence of Herba Houttuyniae （HH）distillate on the urine protein, urinary albumin, renal connective tissue growth factor （CTGF）, adiponectin （APN）, and monocyte chemoattractant protein （MCP-1）of rats with type 2 diabetes mellitus （T2DM）. Methods The rat model of T2DM was induced by one-time intraperitoneal injection of small-dosage of streptozotocin （STZ）and feeding with high glucose and lipid, and then treated with HH for 8 weeks. After treatment, the changes of biochemical parameters such as 24-h urinary protein, urinary albumin, the renal CTGF, APN and MCP-1 were observed. Results After treatment with HH distillate, the symptoms of polydipsia, polyphagia and emaciation were relieved, and the 24-h urinary protein, urinary albumin, and renal CTGF and MCP-1 expression levels were decreased in T2DM rats, but the APN level remained unchanged. Conclusion The HH distillate can relieve the renal pathological changes of T2DM rats, and the mechanism is probably related with the reduction of urinary protein and Alb level and with the decrease of renal MCP-1 and CTGF expression level.