平肺口服液抑制肺成纤维细胞生长和诱导凋亡

Growth Inhibition and Apoptosis Induction of Human Lung Fibroblasts by Pingfei Oral Liquid

目的采用血清药理学方法,研究平肺口服液对人胚肺成纤维细胞增殖及诱导细胞凋亡的作用。方法平肺口服液10g/kg和20g/kg分别灌服大鼠,第1～7天每日一次,第8～10天每日两次,于第11天早上末次灌服后2h腹主动脉无菌采血制备含药血清。采用噻唑蓝(MTT)比色法和流式细胞术分别检测平肺口服液含药血清对人胚肺成纤维细胞(CCC-HPF-1)增殖、细胞凋亡和细胞周期的影响。结果平肺口服液20g/kg灌服所得大鼠含药血清作用96h和120h分别能抑制CCC-HPF-1细胞增殖(P<0.01);流式细胞术检测表明该含药血清作用96h能诱导CCC-HPF-1细胞发生凋亡(P<0.05)和G1/G0期阻滞(P<0.05)。结论平肺口服液能抑制肺成纤维细胞增殖,诱导细胞凋亡以及使细胞周期阻滞于G1/G0期,这可能有助于其预防放射性肺损伤。

Objective To determine the effects of Pingfei Oral Liquid （PFOL）,which is a Traditional Chinese medicine（TCM）prescription, on the growth, apoptosis and cell cycle of human lung fibroblasts. Methods Serum con- taining PFOL was prepared by serologic pharmacology method . PFOL was administered orally to SD rats at doses of 10 g/kg and 20 g/kg respectively, once a day from the 1st to 7 th day, and twice a day from the 8th to 10th day. Blood was obtained from abdominal aorta aseptically at the l lth day 2 hours after the last morning administration and then the serum was separated. The proliferation of human lung fibroblasts （CCC-HPF-1）after exposure to the serum was mea- sured by MTT assay. The flow cytometry was used to detect the apoptosis and cell cycle of CCC-HPF-1. Results The growth of CCC-HPF-1 could be suppressed at the 96 h and 120 h after exposure to the serum from rats treated with 20 g/kg PFOL （P 〈 0.01）. The cells were also induced apoptosis （P 〈 0.05）and arrested at G1/G0 phase at 96 h after exposure to the serum （P 〈 0.05）. Conclusion PFOL could inhibit proliferation and induce apoptosis of lung fibrob- lasts, which may contribute to its protective effect on radiation-induced lung injury.