姜黄素对大鼠血瘀性脑缺血模型全血黏度、脑匀浆ATP酶及脑组织形态的影响

Effect of Curcumine on Whole Blood Viscosity,Cerebral Homogenate ATP Enzyme and Morphology of Brain Tissue in Rat Cerebral Ischemia Model with Blood Stasis

目的探讨姜黄素对大鼠血瘀性脑缺血模型影响的特点。方法采用注射地塞米松复制血瘀模型,姜黄素混悬液给血瘀模型大鼠连续灌服10d,于第11天给药物后1h,大鼠两侧颈总动脉结扎30min,造脑缺血模型;取血测全血黏度;取大鼠脑,一半脑组织用生理盐水制脑匀浆,测脑匀浆ATP水平;另一半脑组织固定、切片,观察脑组织的病理变化。结果肌肉注射地塞米松可成功复制血瘀模型,结扎双侧颈总动脉可成功复制脑缺血模型。与模型组比较,姜黄素可显著降低血瘀性脑缺血模型全血黏度,显著提高脑匀浆Na+-K+-ATP、Mg2+-ATP、Ca2+-ATP和Ca2+-Mg2+-ATP酶活性,显著改善模型所致脑神经细胞和胶质细胞的萎缩。结论姜黄素可显著改善大鼠血瘀性脑缺血模型全血黏度和脑匀浆ATP酶及脑组织形态。

Objective To explore the effect of curcumine on rat cerebral ischemia model of blood stasis. Methods The blood-stasis rat model was established by injection of dexamethasone, then was given gastric infusion curcumine suspension for 10 continuous days. One hour after administration on the llth day, we made ligation of the bilatory common carotid arteries of rats for 30 min to induce cerebral ischemia rat model. The rat blood sample was used for the detection of whole blood viscosity, and the brain was taken out for the observation of cerebral homogenate ATP and the pathological changes of brain tissue. Results The blood-stasis rats model was established successfully by intramuscular injection of dexamethasone, and cerebral ischemia rats model was established successfully by ligation of bilateral common carotid artery. Compared with the model group, curcumine could significantly reduce the whole blood viscosity of blood-stasis rats model of cerebral ischemia, and significantly increase the activity of Na＋-K＋-ATP, Mg2＋- ATP, Ca2＋-ATP and Ca2＋-Mg2＋-ATP in brain homogenate, significant relieve the atrophy of nerve cells and glial ceils of the model rats. Conclusion Curcumine can significantly improve the whole blood viscosity, ATP activity in the brain homogenate and morphological changes of brain tissue in rats model of blood stasis .