柱前衍生RP-HPLC法测定四角蛤蜊中牛磺酸的含量

Determination of Taurine in Mactra Veneriformis by Pre-column Derivatization RP-HPLC

目的柱前衍生HPLC法测定四角蛤蜊中牛磺酸的含量。方法使用超声法提取四角蛤蜊中牛磺酸,样品提取液与牛磺酸标准液经异硫氰酸苯脂衍生化后,采用Kromasil C18柱(4.6mm×250mm,5μm)分离,以醋酸钠-水-甲醇为流动相,在254nm检测牛磺酸衍生产物。结果样品提取液与对照品存在相对应的牛磺酸色谱峰。方法学考察表明牛磺酸进样浓度在10.04～50.20μg·mL-1范围内与其峰面积有良好的线性关系(r=0.9998),加样回收率为97.95%(n=9),RSD为1.41%。结论该方法可以准确测定四角蛤蜊中牛磺酸的含量,且灵敏度高,重现性好。不同季节四角蛤蜊中牛磺酸的含量在10.56～20.91mg/g之间,6月份含量达到最高值。

Objective To determine the content of taurine in Mactra veneriformis by pre-column derivation HPLC. Methods Ultrasound method was used for the extraction of taurine from Mactra veneriformis. In alkaline condition, amino acids reacted with phenyl isothiocyanate and resulted in the derivatives of benzene isothiocyanate-amino acid complex. Kromasil Cls column （4.6 mm ×250 mm, 5 μm） was used and the absorbance at 254 nm was measured. The mobile phase consisted of sodium acetate solution, water and methanol. Results Corresponding chromatographic peaks of taurine were obtained in extract sample and the control. The linear range of taurine was from 10.04 μg·mL-1 to 50.20 μg ·mL-1, and the recovery, was 97.95 %（RSD=1.41%, n=9）. Conclusion This method is simple, accu- rate and reproducible. The seasonal variation range of taurine level is from 10.56 mg/g to 20.91 rag/g, and the taurine level arrived to the peak in June.