辅助腹腔复苏治疗失血性休克大鼠的研究

Adjunct intraperitoneal resuscitation therapy for hemorrhagic shock of rats

目的探讨辅助腹腔复苏对失血性休克的治疗作用。方法制作失血性休克大鼠模型,平均动脉压(MAP)降为40mmHg(1mmHg=0.133kPa)并持续90min开始行传统方法或辅助腹腔复苏,观察不同复苏方法对休克大鼠MAP的影响;于复苏后3h处死部分大鼠,取回肠组织,光镜下观察肠黏膜绒毛形态,评价肠黏膜损伤程度;观察不同复苏方法对休克大鼠存活时间的影响。结果与传统复苏方法相比,辅助腹腔复苏能显著提高创伤失血性休克大鼠的MAP(P<0.05)和显著减轻休克大鼠肠黏膜绒毛的损伤(P<0.01)以及降低休克大鼠的死亡率和延长存活时间。结论辅助腹腔复苏能对失血性休克有较好的疗效。

Objective To explore the effect of adjunct intraperitoneal resuscitation on hemorrhagic shock（HS） of rats.Methods A HS model of rats was established.Conventional resuscitation（CR） or CR plus adjunct intra-peritoneal resuscitation（IR） was performed when the mean aortic pressure（MAP） remained 40mmHg for 90min to observe the effect of different resuscitation methods on MAP.Three hours after resuscitation, some rats were killed with their ileum mucosa removed to observe its villus morphology under a light microscope.The degree of ileum mucosa injury was evaluated and the effect of different resuscitation methods on survival time of other rats was observed.Results Compared with CR, IR could significantly improve the MAP（P0.05）, attenuate the ileum mucosal villus injury（P0.01）, prolong the survival time, and decrease the mortality of rats with HS.Conclusion Adjunct intraperitoneal resuscitation therapy can be a more effective way for HS.