气相色谱法测定人工麝香口崩片中麝香酮的含量

Determination of Muscone in the Orally Disintegrating Tablets of Artificial-musk by GC

目的建立人工麝香口崩片中麝香酮的含量测定方法。方法气相色谱法,样品以甲醇超声提取,Varian-3800型气相色谱仪,色谱柱:Cp-sil 8 cb low bleed/ms(30 m×0.25 mm,0.25μm);程序升温:160℃～250℃;检测器:氢火焰离子化检测器(FID),280℃,进样口温度260℃;载气为N_2,流速1.06mL/min,分流比10:1。结果麝香酮在56.7～226.8μg/mL线性良好(r=0.999 7),平均回收率100.14%,RSD=1.68%。结论本方法简便,快速、准确、重现性好,可作为本制剂的质量控制方法。

Objective To establish a method for the determination ofmuscone in the orally disintegrating tablets of artificial-musk by GC. Methods The sample was extracted by supersonic extraction with methanol. The contents were assayed by a Cp-sil 8 cb low bleed/ms （ 30 m×0.25 mm,0.25 um ） column with temperature programmed condition in which the initial temperature was 140℃ and the final temperature was 250℃, and it was detected by hydrogen flame ionization （FID） at 260℃ with nitrogen as the carrier gas of 1.06 mL/min and the split ratio was 10：1. The injector temperature was 250.%. Results The standard curve ofmuscone was linear over the range of 56.7-226.8 ug/mL（r=0.999 7）.The average recovery was 100.14% with RSD of 1.68%. Conclusions This method is proved to be convenient, fast and accurate. It can be reliable for the quality control of the orally disintegrating tablets of artificialmusk.