摘要
根据水稻β-1,4-糖苷酶(korrigan)基因的保守区序列设计引物,以毛竹cDNA为模板,采用PCR方法,成功扩增出1个含有完整阅读框架的cDNA序列,长度为2191bp,共编码617个氨基酸,将其命名为PeKOR基因。其氨基酸序列分析的结果表明,PeKOR与其他β-1,4-糖苷酶有较高的同源性,同水稻序列相似性高达91%,且其序列具有典型的Glycosyl hydrolase9super family结构域,推测此PeKOR为毛竹β-1,4-糖苷酶基因。在竹笋中采用半定量方法研究该基因的表达情况,结果表明该基因在高温条件下表达量较低温条件下明显升高。
A beta-1,4-glycosidase protein gene from phyUostachys edulis,named as PeKOR,was cloned through PCR using primers designed according to the beta-1,4-glycosidase protein genes conserved region of other plant. The coding region of the genomic clone of PeKOR is continuous. The cDNA of PeKORcontains an open reading frame of 2 191 bp and codes for a protein of 617 aa. The database search using the amino acid sequence as query showed high homology to several beta-1,4-glycosidase proteins,especially the PeKOR sequence showed the maximum homology (91% identity)to Oryza sativa subspecies beta-1,4-glycosidase protein gene) , and it has a typically conserved domain of Glycosyl hydrolase 9 super family. Therefore we predicted that the PeKOR could be an beta-1,4-glyeosidase genein bamboo. The result of semiquantitative RT-PCR in bamboo shoots showed that expression of PeKOR gene was regulated by temperature, and the expression level on high temperature is larger than the one on low temperature.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第3期104-108,共5页
Biotechnology Bulletin
关键词
毛竹
β—1
4-糖苷酶克隆表达分析
Phyllostachys pubescens Beta-1,4 glycosidase protein Cloning Expression analysis