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HSV-2 LAT ORF2在Vero细胞中的表达及对细胞的作用 被引量：2

HSV-2 LAT ORF2 Expression in Vero Cells and Effects on Cells
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摘要 旨在研究人单纯疱疹病毒2型(HSV-2)潜伏相关转录体(LAT)开放读码框2(ORF2)对体外培养的非洲绿猴肾细胞(Vero)形态和活性的作用。将构建好的带绿色荧光蛋白(EGFP)标签的HSV-2LAT ORF2真核表达载体pEGFP-ORF2,转染vero细胞,荧光显微镜观测细胞形态的改变和MTT法进行活性分析。结果:显示,HSV-2LATORF2诱导细胞形态发生明显变化,绿色荧光蛋白在细胞的定位也发生了改变,细胞活性降低。由此证实,HSV-2LAT ORF2对细胞有损伤作用,为阐明HSV-2LATORF2的功能提供了资料。 It was to explore the effect of HSV-2 LAT ORF2 on cytomorphology and cell activity in vitro. Eukaryotic expressive veetors of EGFP-ORF2 fusion protein named pEGFP-ORF2 was transfected into Vero cells by liposome transfection reagent,morphogenesis were observed by fluorescence microscopy and cell activity were analyzed by MTT. Results that HSV-2 LAT ORF2 had evident change in cytomorphology,the location of fusion protein of EGFP and HSV-2 LAT ORF2 in cells has changed,and MTT analysis revealed the cell activity decreased. Therefore,it proved that HSV-2 LAT ORF2 has impairment on cells, and these results may shed light on the fur ther study of the function of HSV-2.
作者 白利利 杨慧兰
机构地区 华南理工大学生物科学与工程学院 广州军区广州总医院皮肤科
出处 《生物技术通报》 CAS CSCD 北大核心 2010年第3期160-163,共4页 Biotechnology Bulletin
基金 国家自然科学基金资助项目(30972666) 全军医药卫生科研基金资助项目(06J008)
关键词 HSV-2 LAT ORF2绿色荧光蛋白 VERO细胞 HSV-2 LAT ORF2 Green fluorescent protein Vero cell line
分类号 R752.1 [医药卫生—皮肤病学与性病学]
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参考文献7

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共引文献2

同被引文献13

  • 1陈红,钱坤,张苏明.不同转染方法及GFP表达载体对小鼠胚胎干细胞转染效率的比较[J].华中科技大学学报（医学版）,2006,35(4):536-537. 被引量：6
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生物技术通报
2010年 第3期

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