膜TNF-α在小鼠Con A-LAK细胞介导的MHC非限制性杀瘤效应中的作用

The Role of Membrane TNF-a in Murine Con A-LAK Cell Mediated MHC Non-restricted Tumoricidal Effect

本文研究Con A预刺激小鼠LAK细胞(Con A-LAK)的体外杀瘤活性,探讨膜TNF-α在其杀瘤效应中的作用.结果表明.Con A-LAK可杀伤NK敏感的YAC-1、TNF敏感的L929及H-2低/无表达的HCa-F等肿瘤靶细胞;其杀瘤作用主要是直接杀伤,而效应细胞表面配体与靶细胞表面受体相互作用介导的非分泌途径起着重要的作用;Con A-LAK表达膜TNF-α,后者参与杀伤TNF敏感的肿瘤靶细胞,可能是介导MHC非限制性、抗原非特异性杀瘤效应的重要效应分子之一.

This essay investigated the in vitro tumoricidal activity of Con A primed murine LAK cells (Con A-LAK), discussed the role of mTNF-α in the killing effect. The results showed that Con A-LAK cells could kill NK sensitive YAC-1, TNF sensitive L929 and H-2 low/non expression HCa-F target cells; The cytotoxicitity of Con A-LAK cells was performed by direct contact, the non-secretary pathway mediated by interaction of surface ligands of effector cells and receptors of target cells play an important role; Con A-LAK cells expressed mTNF-α, which participated in the killing of TNF sensitive tumor cells and might be one of the important effector molecules in MHC non-restricted, antigen non-specific cvtotoxicitv.