热疗对肺癌A549细胞P-糖蛋白表达影响的实验研究

Influence of hyperthermia on expression of P-gp in lung cancer A549 cell

目的研究不同温度、不同加热时间下热疗对肺癌A549细胞P-糖蛋白(P-gp)表达的影响,为肺癌的热疗和热化疗提供理论依据。方法体外复苏与培养肺癌A549细胞;对照组37℃下培养,实验组按不同时间、不同温度分组水浴加热肺癌A549细胞后培养24 h,采用流式细胞术(FCM)观察热处理后的肺癌A549细胞株P-gp表达的变化。结果流式细胞术显示各热疗组A549细胞的P-gp的表达率均较对照组下降,且P-gp的表达率与加热的时间和温度均呈负相关。结论热疗可以抑制P-gp的表达,可能是逆转肿瘤细胞多药耐药的一种手段。

Objective To investigate the influence of hyperthermia on the expression of P-glyeoprotein （P-gp） in lung cancer A549 cell by different temperature and heating time for providing the academic evidence in hyperthermia and thermochemotherapy for lung cancer. Methods To resuscitate and culture the A549 cells in vitro. Control group：AS49 cells were cultivated at 37 ℃. Ex perimental group：A549 cells were cultivated for 24 h after heated by different temperature and heating time. The flow eytometry （FCM） was adopted to observe the influence on the P gp expression of A549 cell treated by thermotherapy. Results The FCM showed hyperthermia could cause reduction of P-gp expression in A549 cell and the expression of P-gp is negative correlation with the heating temperature and heating time. Conclusion Hyperthermia could cause reduction of P gp expression in A549 cell. The re sults suggest that hyperthermia could be used as a method to overcome multidrug resistance.